Ferreres Federico, Sousa Carla, Valentão Patrícia, Andrade Paula B, Seabra Rosa M, Gil-Izquierdo Angel
Research Group on Quality, Safety and Bioactivity of Plant Foods, Department of Food Science and Technology, CEBAS (CSIC), P.O. Box 164, 30100 Campus Univ. Espinardo, Murcia, Spain.
J Agric Food Chem. 2007 Dec 12;55(25):10187-93. doi: 10.1021/jf072119y. Epub 2007 Nov 15.
The flavonoids present in passion fruit (Passiflora edulis) leaves were identified by a high-performance liquid chromatography-diode array detection-tandem mass spectrometry (HPLC-DAD-MS/MS) method. Sixteen apigenin or luteolin derivatives were characterized, which included four mono-C-glycosyl, eight O-glycosyl- C-glycosyl, and four O-glycosyl derivatives. With the exceptions of C-hexosyl luteolin and C-hexosyl apigenin, all the compounds exhibited a deoxyhexose moiety. Moreover, the uncommon C-deoxyhexosyl derivatives of luteolin and apigenin have been identified for first time in P. edulis by HPLC-DAD-MS/MS. The antioxidative capacity of passion fruit leaves was checked against DPPH radical and several reactive oxygen species (superoxide radical, hydroxyl radical, and hypochlorous acid), revealing it to be concentration-dependent, although a pro-oxidant effect was noticed for hydroxyl radical.
采用高效液相色谱-二极管阵列检测-串联质谱（HPLC-DAD-MS/MS）法对西番莲（Passiflora edulis）叶片中的黄酮类化合物进行了鉴定。表征了16种芹菜素或木犀草素衍生物，其中包括4种单-C-糖基、8种O-糖基-C-糖基和4种O-糖基衍生物。除C-己糖基木犀草素和C-己糖基芹菜素外，所有化合物均含有一个脱氧己糖部分。此外，通过HPLC-DAD-MS/MS首次在西番莲中鉴定出木犀草素和芹菜素罕见的C-脱氧己糖基衍生物。检测了西番莲叶片对DPPH自由基和几种活性氧（超氧自由基、羟基自由基和次氯酸）的抗氧化能力，结果表明其具有浓度依赖性，不过对羟基自由基有促氧化作用。
