Schaufelberger D E, Koleck M P, Beutler J A, Vatakis A M, Alvarado A B, Andrews P, Marzo L V, Muschik G M, Roach J, Ross J T
Chemical Synthesis and Analysis Laboratory, PRI/DynCorp, National Cancer Institute--Frederick Cancer Research and Development Center, Maryland 21702.
J Nat Prod. 1991 Sep-Oct;54(5):1265-70. doi: 10.1021/np50077a004.
A novel process was designed for the large-scale isolation of bryostatin 1 from the bryozoan Bugula neritina L. in order to obtain multigram quantities of highly pure material for formulation studies, preclinical toxicology, and clinical trials in cancer patients. Multigram quantities of bryostatin 1 were obtained from a collection of approximately 10,000 gallons of wet animal. A phorbol dibutyrate (PDBu) receptor binding assay and hplc with photodiode array detection were used for the design, validation, and control of the isolation process.
设计了一种新方法，用于从苔藓虫类的美丽琥珀苔虫中大规模分离苔藓抑素1，以便获得数克高纯度物质，用于制剂研究、临床前毒理学研究以及癌症患者的临床试验。从大约10,000加仑湿动物体中获得了数克苔藓抑素1。采用佛波醇二丁酸酯（PDBu）受体结合测定法和带光电二极管阵列检测的高效液相色谱法对分离过程进行设计、验证和控制。
