由限制性核酸内切酶产生的BK病毒DNA片段的图谱绘制与排序
Mapping and ordering of fragments of BK virus DNA produced by restriction endonucleases.
作者信息
Freund J, di Mayorca G, Subramanian K N
出版信息
J Virol. 1979 Mar;29(3):915-25. doi: 10.1128/JVI.29.3.915-925.1979.
Abstract
A total of 51 restriction sites were recognized within the BK virus genome by the combination of 10 different restriction endonucleases. These sites were mapped and oriented relative to one another as well as to the five fragments generated by the digestion of BK virus DNA with HindIII and EcoRI. The result was a comprehensive physical map suitable for in-depth characterization of the functions of BK virus at the molecular level.
摘要
通过10种不同的限制性内切酶组合，在BK病毒基因组中总共识别出51个限制性位点。这些位点相对于彼此以及相对于用HindIII和EcoRI消化BK病毒DNA产生的五个片段进行了定位和定向。结果得到了一张全面的物理图谱，适用于在分子水平上深入表征BK病毒的功能。
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