JC病毒DNA的限制性内切酶切割图谱。
Restriction endonuclease cleavage map of the DNA of JC virus.
作者信息
Martin J D, Frisque R J, Padgett B L, Walker D L
出版信息
J Virol. 1979 Mar;29(3):846-55. doi: 10.1128/JVI.29.3.846-855.1979.
Abstract
A physical map of the sites cleaved by the following restriction endonucleases was derived for the DNA of JC virus, a human polyomavirus: EcoRI, HpaI, and PstI (one site each); HindII (four sites); and HindIII (three sites). By agarose gel electrophoresis of fragmented DNA, the size of full-length DNA of JC virus was estimated to be 5,125 +/- 105 base pairs (98 +/- 2% of the length of simian virus 40 DNA).
摘要
已绘制出人类多瘤病毒JC病毒DNA被以下限制性内切酶切割位点的物理图谱：EcoRI、HpaI和PstI（各一个位点）；HindII（四个位点）；以及HindIII（三个位点）。通过对片段化DNA进行琼脂糖凝胶电泳，估计JC病毒全长DNA的大小为5125 ± 105个碱基对（占猿猴病毒40 DNA长度的98 ± 2%）。
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