Institute for Molecular Cardiovascular Research (IMCAR), RWTH Aachen University, Aachen, Germany.
XLIM Research Institute, Limoges University, CNRS, Limoges, France.
Mol Imaging Biol. 2023 Feb;25(1):3-17. doi: 10.1007/s11307-021-01665-2. Epub 2021 Nov 15.
In recent years, the demand for non-destructive deep-tissue imaging modalities has led to interest in multiphoton endoscopy. In contrast to bench top systems, multiphoton endoscopy enables subcellular resolution imaging in areas not reachable before. Several groups have recently presented their development towards the goal of producing user friendly plug and play system, which could be used in biological research and, potentially, clinical applications. We first present the technological challenges, prerequisites, and solutions in two-photon endoscopic systems. Secondly, we focus on the applications already found in literature. These applications mostly serve as a quality check of the built system, but do not answer a specific biomedical research question. Therefore, in the last part, we will describe our vision on the enormous potential applicability of adult two-photon endoscopic systems in biological and clinical research. We will thus bring forward the concept that two-photon endoscopy is a sine qua non in bringing this technique to the forefront in clinical applications.
近年来，对无损深层组织成像模式的需求促使人们对多光子内窥镜产生了兴趣。与台式系统相比，多光子内窥镜能够在以前无法到达的区域实现亚细胞分辨率成像。最近，有几个研究小组展示了他们在开发用户友好型即插即用系统方面的进展，该系统可用于生物研究，并有可能用于临床应用。我们首先介绍了双光子内窥镜系统的技术挑战、前提条件和解决方案。其次，我们重点介绍了文献中已经发现的应用。这些应用主要作为构建系统的质量检查，但并不能回答特定的生物医学研究问题。因此，在最后一部分，我们将描述我们对成人双光子内窥镜系统在生物和临床研究中巨大潜在应用的看法。我们将提出这样一个概念，即双光子内窥镜是将该技术推向临床应用前沿的必要条件。
