Tjøtta E, Hungnes O, Grinde B
Department of Virology, National Institute of Public Health, Oslo, Norway.
J Med Virol. 1991 Dec;35(4):223-7. doi: 10.1002/jmv.1890350402.
A recently developed assay for measuring infectious HIV-1 particles was used to determine the stability of the virus under various storage conditions as well as the effect of commonly used disinfectants. At the optimum pH of 7.1 the half life of the virus ranged from approx. twenty-four hours at 37 degrees C to no significant loss over 6 months at -75 degrees C. Drying the virus on a glass surface or freezing caused a 5-12 fold and 4-5 fold decrease of activity, respectively. The dried preparations, however, were about as stable as when stored in a buffered solution. A solution of iodine and detergent (2% Jodopax) was the only disinfectant examined which removed all detectable HIV-1 activity. Isopropanol and ethanol were more potent than acetone; however, all three solvents left some viable particles after a 30 min treatment with 70% solutions.
一种最近开发的用于测量传染性HIV-1颗粒的检测方法被用于确定该病毒在各种储存条件下的稳定性以及常用消毒剂的效果。在最佳pH值7.1时，病毒的半衰期范围从37摄氏度下约24小时到-75摄氏度下6个月内无显著活性损失。在玻璃表面干燥病毒或冷冻分别导致活性降低5至12倍和4至5倍。然而，干燥制剂的稳定性与储存在缓冲溶液中时大致相同。碘和洗涤剂溶液（2%碘多帕）是所检测的唯一能去除所有可检测到的HIV-1活性的消毒剂。异丙醇和乙醇比丙酮更有效；然而，用70%溶液处理30分钟后，这三种溶剂都留下了一些活的颗粒。
