Peng Junping, Yang Li, Yang Fan, Yang Jian, Yan Yongliang, Nie Huan, Zhang Xiaobing, Xiong Zhaohui, Jiang Yan, Cheng Fan, Xu Xingye, Chen Shuxia, Sun Lilian, Li Weijun, Shen Yan, Shao Zhujun, Liang Xiaofeng, Xu Jianguo, Jin Qi
State Key Laboratory for Molecular Virology and Genetic Engineering, National Institute for Viral Disease Control and Prevention, Beijing 100052, China.
Genomics. 2008 Jan;91(1):78-87. doi: 10.1016/j.ygeno.2007.10.004. Epub 2007 Nov 26.
Ten outbreaks of a new serogroup C meningococcal disease emerged during 2003-2005 in China. The multilocus sequence typing results indicated that unique sequence type 4821 clone meningococci were responsible for these outbreaks. Herein, we determined the entire genomic DNA sequence of serogroup C isolate 053442, which belongs to ST-4821. Comparison of 053442 gene contents with other meningococcal genomes shows that they have similar characteristics, including thousands of repetitive elements and simple sequence repeats, numerous phase-variable genes, and similar virulence-related factors. However, many strain-specific regions were found in each genome. We also present the results of a genomic comparison of 28 ST-4821 complex isolates that were isolated from different serogroups using comparative genomic hybridization analysis. Genome comparison between the newly emerged hyperinvasive isolates belonging to different serogroups will further our understanding of their respective pathogenetic mechanisms.
2003年至2005年期间，中国出现了10起新型C群脑膜炎球菌病疫情。多位点序列分型结果表明，独特序列型4821克隆脑膜炎球菌是这些疫情的罪魁祸首。在此，我们测定了属于ST-4821的C群分离株053442的全基因组DNA序列。将053442的基因内容与其他脑膜炎球菌基因组进行比较，结果显示它们具有相似的特征，包括数千个重复元件和简单序列重复、众多相变基因以及相似的毒力相关因子。然而，在每个基因组中都发现了许多菌株特异性区域。我们还展示了使用比较基因组杂交分析对从不同血清群分离出的28株ST-4821复合分离株进行基因组比较的结果。对属于不同血清群的新出现的高侵袭性分离株进行基因组比较，将有助于我们进一步了解它们各自的致病机制。
