Jin Kunlin, Mao Xiao Ou, Xie Lin, Khan Adil A, Greenberg David A
Buck Institute for Age Research, 8001 Redwood Boulevard, Novato, CA 94945, USA.
Neurosci Lett. 2008 Jan 10;430(2):135-7. doi: 10.1016/j.neulet.2007.10.031. Epub 2007 Nov 4.
Neuroglobin (Ngb) is a novel vertebrate globin expressed principally in neurons. Ngb expression is induced by hypoxia and ischemia, and Ngb protects neurons against these insults. The mechanism of Ngb's protective action is unknown, but its ability to bind NO suggests that NO scavenging might be involved. To test this hypothesis, we treated wild type and Ngb-transfected HN33 (mouse hippocampal neuronxN18TG2 neuroblastoma) cells with NO donors and compared their sensitivity to NO-induced cell death. Ngb overexpression shifted concentration-toxicity curves to the right, indicating reduced susceptibility to NO or is metabolites. The results suggest that the ability of Ngb to neutralize the neurotoxic effects of reactive nitrogen species may be an important contributor to its neuroprotective properties.
神经球蛋白（Ngb）是一种主要在神经元中表达的新型脊椎动物球蛋白。Ngb的表达由缺氧和缺血诱导，并且Ngb可保护神经元免受这些损伤。Ngb保护作用的机制尚不清楚，但其结合NO的能力表明可能涉及清除NO。为了验证这一假设，我们用NO供体处理野生型和Ngb转染的HN33（小鼠海马神经元×N18TG2神经母细胞瘤）细胞，并比较它们对NO诱导的细胞死亡的敏感性。Ngb过表达使浓度-毒性曲线右移，表明对NO或其代谢产物的敏感性降低。结果表明，Ngb中和活性氮物种神经毒性作用的能力可能是其神经保护特性的重要因素。
