Goodfriend T L, Pedersen T L, Grekin R J, Hammock B D, Ball D L, Vollmer A
William S. Middleton Memorial Veterans Hospital, University of Wisconsin-Madison, 2500 Overlook Terrace, Madison, WI 53705, USA.
Prostaglandins Leukot Essent Fatty Acids. 2007 Nov-Dec;77(5-6):363-6. doi: 10.1016/j.plefa.2007.10.012. Epub 2007 Nov 26.
We measured 16 nonesterified oxygenated fatty acid derivatives (oxylipids) in plasmas from seven human subjects. Two arterial samples from each subject were analyzed, drawn approximately 2h apart. We observed a marked increase in levels of most oxylipids in the second sample, as high as 470-fold. Between the first and second samples, subjects received approximately 800-1000 IU of heparin to prevent clotting in intravascular catheters. We postulate that heparin activated lipoprotein lipases, which, in turn, released oxylipids from triglycerides and phospholipids in plasma lipoproteins. Some of that lipolysis may have occurred during sample storage. Measurements of nonesterified lipids in human plasma may be distorted if heparin is administered to subjects before blood is drawn and if lipase inhibitors are omitted from stored samples.
我们检测了7名人类受试者血浆中的16种非酯化氧化脂肪酸衍生物（氧化脂质）。对每位受试者的两份动脉样本进行了分析，样本采集时间间隔约为2小时。我们观察到，在第二个样本中，大多数氧化脂质的水平显著升高，高达470倍。在采集第一个和第二个样本期间，受试者接受了约800 - 1000国际单位的肝素，以防止血管内导管凝血。我们推测，肝素激活了脂蛋白脂肪酶，进而从血浆脂蛋白中的甘油三酯和磷脂中释放出氧化脂质。部分脂解作用可能发生在样本储存期间。如果在采血前给受试者注射肝素，且在储存样本时未添加脂肪酶抑制剂，那么人体血浆中非酯化脂质的测量结果可能会失真。
