• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

端粒锚定在核膜周边需要芽殖酵母中含Sad1-UNC-84结构域的蛋白Mps3。

Telomere anchoring at the nuclear periphery requires the budding yeast Sad1-UNC-84 domain protein Mps3.

作者信息

Bupp Jennifer M, Martin Adriana E, Stensrud Elizabeth S, Jaspersen Sue L

机构信息

Stowers Institute for Medical Research, Kansas City, MO 64110, USA.

出版信息

J Cell Biol. 2007 Dec 3;179(5):845-54. doi: 10.1083/jcb.200706040. Epub 2007 Nov 26.

DOI:10.1083/jcb.200706040
PMID:18039933
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2099192/
Abstract

Positioning of telomeres at the nuclear periphery can have dramatic effects on gene expression by establishment of heritable, transcriptionally repressive subdomains. However, little is known about the integral membrane proteins that mediate telomere tethering at the nuclear envelope. Here, we find a previously unrecognized function for the Saccharomyces cerevisiae Sad1-UNC-84 domain protein Mps3 in regulating telomere positioning in mitotic cells. Our data demonstrate that the nucleoplasmic N-terminal acidic domain of Mps3 is not essential for viability. However, this acidic domain is necessary and sufficient for telomere tethering during S phase and the silencing of reporter constructs integrated at telomeres. We show that this is caused by the role of the Mps3 acidic domain in binding and localization of the silent information regulator protein Sir4 to the nuclear periphery. Thus, Mps3 functions as an integral membrane anchor for telomeres and is a novel nuclear receptor for the Sir4 pathway of telomere tethering and gene inactivation.

摘要

端粒定位于核周边可通过建立可遗传的转录抑制亚结构域对基因表达产生显著影响。然而，对于介导端粒在核膜上锚定的整合膜蛋白却知之甚少。在此，我们发现酿酒酵母Sad1-UNC-84结构域蛋白Mps3在调节有丝分裂细胞中端粒定位方面具有先前未被认识的功能。我们的数据表明，Mps3的核质N端酸性结构域对细胞活力并非必需。然而，该酸性结构域对于S期的端粒锚定以及整合在端粒处的报告基因构建体的沉默是必要且充分的。我们表明，这是由Mps3酸性结构域在将沉默信息调节蛋白Sir4结合并定位到核周边中的作用所导致的。因此，Mps3作为端粒的整合膜锚定物，是端粒锚定和基因失活的Sir4途径的新型核受体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/16aa0507cf9a/jcb1790845f08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/7b982a0acfec/jcb1790845f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/3155f3a688fd/jcb1790845f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/a15ac91a8d75/jcb1790845f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/ea2a3248bcbd/jcb1790845f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/6464ffca3a1a/jcb1790845f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/2bc569661ff6/jcb1790845f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/0eb28cebafb9/jcb1790845f07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/16aa0507cf9a/jcb1790845f08.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/7b982a0acfec/jcb1790845f01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/3155f3a688fd/jcb1790845f02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/a15ac91a8d75/jcb1790845f03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/ea2a3248bcbd/jcb1790845f04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/6464ffca3a1a/jcb1790845f05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/2bc569661ff6/jcb1790845f06.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/0eb28cebafb9/jcb1790845f07.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/82a4/2099192/16aa0507cf9a/jcb1790845f08.jpg

相似文献

1
Telomere anchoring at the nuclear periphery requires the budding yeast Sad1-UNC-84 domain protein Mps3.端粒锚定在核膜周边需要芽殖酵母中含Sad1-UNC-84结构域的蛋白Mps3。
J Cell Biol. 2007 Dec 3;179(5):845-54. doi: 10.1083/jcb.200706040. Epub 2007 Nov 26.
2
Ribosome biogenesis factors bind a nuclear envelope SUN domain protein to cluster yeast telomeres.核膜 SUN 结构域蛋白与核糖体生物发生因子结合，使酵母端粒聚集。
EMBO J. 2011 Aug 5;30(18):3799-811. doi: 10.1038/emboj.2011.267.
3
Yeast telomerase and the SUN domain protein Mps3 anchor telomeres and repress subtelomeric recombination.酵母端粒酶与SUN结构域蛋白Mps3锚定端粒并抑制亚端粒重组。
Genes Dev. 2009 Apr 15;23(8):928-38. doi: 10.1101/gad.1787509.
4
The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to connect the spindle pole body with the nuclear envelope.Mps3中的Sad1-UNC-84同源结构域与Mps2相互作用，将纺锤体极体与核膜连接起来。
J Cell Biol. 2006 Aug 28;174(5):665-75. doi: 10.1083/jcb.200601062. Epub 2006 Aug 21.
5
TFIIIC localizes budding yeast ETC sites to the nuclear periphery.TFIIIC 将酵母的 ETC 位点定位于核周。
Mol Biol Cell. 2012 Jul;23(14):2741-54. doi: 10.1091/mbc.E11-04-0365. Epub 2012 Apr 11.
6
Separation of silencing from perinuclear anchoring functions in yeast Ku80, Sir4 and Esc1 proteins.酵母Ku80、Sir4和Esc1蛋白中沉默功能与核周锚定功能的分离。
EMBO J. 2004 Mar 24;23(6):1301-12. doi: 10.1038/sj.emboj.7600144. Epub 2004 Mar 11.
7
Mps2 links Csm4 and Mps3 to form a telomere-associated LINC complex in budding yeast.Mps2 将 Csm4 和 Mps3 连接起来，在出芽酵母中形成与端粒相关的 LINC 复合物。
Life Sci Alliance. 2020 Sep 23;3(12). doi: 10.26508/lsa.202000824. Print 2020 Dec.
8
Genetic analysis of Mps3 SUN domain mutants in Saccharomyces cerevisiae reveals an interaction with the SUN-like protein Slp1.酵母 Mps3 SUN 结构域突变体的遗传学分析揭示了与 SUN 样蛋白 Slp1 的相互作用。
G3 (Bethesda). 2012 Dec;2(12):1703-18. doi: 10.1534/g3.112.004614. Epub 2012 Dec 1.
9
Esc1, a nuclear periphery protein required for Sir4-based plasmid anchoring and partitioning.Esc1，一种基于Sir4的质粒锚定和分配所需的核周蛋白。
Mol Cell Biol. 2002 Dec;22(23):8292-301. doi: 10.1128/MCB.22.23.8292-8301.2002.
10
Localization of yeast telomeres to the nuclear periphery is separable from transcriptional repression and telomere stability functions.酵母端粒定位于核周边与转录抑制和端粒稳定性功能可分离。
Mol Cell. 2001 Jul;8(1):189-99. doi: 10.1016/s1097-2765(01)00287-8.

引用本文的文献

1
Yeast centrosomes act as organizing centers to promote Polo kinase-mediated adaptation to persistent DNA damage.酵母中心体作为组织中心，促进Polo激酶介导的对持续性DNA损伤的适应性反应。
Proc Natl Acad Sci U S A. 2025 Jul 8;122(27):e2414426122. doi: 10.1073/pnas.2414426122. Epub 2025 Jul 2.
2
SUN-domain proteins of the malaria parasite are essential for proper nuclear division and DNA repair.疟原虫的SUN结构域蛋白对于正常的核分裂和DNA修复至关重要。
mBio. 2025 Apr 9;16(4):e0021625. doi: 10.1128/mbio.00216-25. Epub 2025 Mar 5.
3
Loss of cytoplasmic actin filaments raises nuclear actin levels to drive INO80C-dependent chromosome fragmentation.

本文引用的文献

1
Functional association of Sun1 with nuclear pore complexes.Sun1与核孔复合体的功能关联。
J Cell Biol. 2007 Aug 27;178(5):785-98. doi: 10.1083/jcb.200704108.
2
The nuclear envelope and transcriptional control.核膜与转录调控。
Nat Rev Genet. 2007 Jul;8(7):507-17. doi: 10.1038/nrg2122. Epub 2007 Jun 5.
3
The nuclear envelope protein Matefin/SUN-1 is required for homologous pairing in C. elegans meiosis.核膜蛋白Matefin/SUN-1是秀丽隐杆线虫减数分裂中同源配对所必需的。
细胞质肌动蛋白丝的丧失会提高核肌动蛋白水平，从而驱动 INO80C 依赖性染色体片段化。
Nat Commun. 2024 Nov 15;15(1):9910. doi: 10.1038/s41467-024-54141-0.
4
DNA methylation-based high-resolution mapping of long-distance chromosomal interactions in nucleosome-depleted regions.基于 DNA 甲基化的核小体缺失区域中长距离染色体相互作用的高分辨率图谱绘制。
Nat Commun. 2024 May 22;15(1):4358. doi: 10.1038/s41467-024-48718-y.
5
The SUN-family protein Sad1 mediates heterochromatin spatial organization through interaction with histone H2A-H2B.SUN 家族蛋白 Sad1 通过与组蛋白 H2A-H2B 的相互作用介导异染色质的空间组织。
Nat Commun. 2024 May 21;15(1):4322. doi: 10.1038/s41467-024-48418-7.
6
A Yeast Mitotic Tale for the Nucleus and the Vacuoles to Embrace.酵母有丝分裂的故事——核与液泡的拥抱。
Int J Mol Sci. 2023 Jun 6;24(12):9829. doi: 10.3390/ijms24129829.
7
Nuclear genome organization in fungi: from gene folding to Rabl chromosomes.真菌中的核基因组组织：从基因折叠到 Rabl 染色体。
FEMS Microbiol Rev. 2023 May 19;47(3). doi: 10.1093/femsre/fuad021.
8
SIR telomere silencing depends on nuclear envelope lipids and modulates sensitivity to a lysolipid.SIR 端粒沉默依赖于核膜脂质，并调节对溶血磷脂的敏感性。
J Cell Biol. 2023 Jul 3;222(7). doi: 10.1083/jcb.202206061. Epub 2023 Apr 12.
9
Loss of SUN1 function in spermatocytes disrupts the attachment of telomeres to the nuclear envelope and contributes to non-obstructive azoospermia in humans.精子细胞中SUN1功能的丧失会破坏端粒与核膜的附着，并导致人类非梗阻性无精子症。
Hum Genet. 2023 Apr;142(4):531-541. doi: 10.1007/s00439-022-02515-z. Epub 2023 Mar 18.
10
The regional sequestration of heterochromatin structural proteins is critical to form and maintain silent chromatin.异染色质结构蛋白的区域性隔离对于形成和维持沉默染色质至关重要。
Epigenetics Chromatin. 2022 Jan 31;15(1):5. doi: 10.1186/s13072-022-00435-w.
Dev Cell. 2007 Jun;12(6):873-85. doi: 10.1016/j.devcel.2007.05.004.
4
SUN1 is required for telomere attachment to nuclear envelope and gametogenesis in mice.SUN1是小鼠端粒附着于核膜及配子发生所必需的。
Dev Cell. 2007 Jun;12(6):863-72. doi: 10.1016/j.devcel.2007.03.018.
5
Nuclear organization of the genome and the potential for gene regulation.基因组的核组织与基因调控潜力。
Nature. 2007 May 24;447(7143):413-7. doi: 10.1038/nature05916.
6
MPS3 mediates meiotic bouquet formation in Saccharomyces cerevisiae.MPS3介导酿酒酵母中的减数分裂花束形成。
Proc Natl Acad Sci U S A. 2007 May 22;104(21):8863-8. doi: 10.1073/pnas.0606165104. Epub 2007 May 10.
7
Transmembrane protein Sun2 is involved in tethering mammalian meiotic telomeres to the nuclear envelope.跨膜蛋白Sun2参与将哺乳动物减数分裂端粒与核膜相连。
Proc Natl Acad Sci U S A. 2007 May 1;104(18):7426-31. doi: 10.1073/pnas.0609198104. Epub 2007 Apr 23.
8
Highway to the inner nuclear membrane: rules for the road.通往内核膜的高速公路：道路规则
Nat Rev Mol Cell Biol. 2007 May;8(5):414-20. doi: 10.1038/nrm2165. Epub 2007 Apr 18.
9
The nuclear envelope and spindle pole body-associated Mps3 protein bind telomere regulators and function in telomere clustering.核膜和纺锤体极体相关的Mps3蛋白结合端粒调节因子并在端粒聚集过程中发挥作用。
Cell Cycle. 2007 Jan 1;6(1):75-9. doi: 10.4161/cc.6.1.3647. Epub 2007 Jan 27.
10
Characterization of the structures involved in localization of the SUN proteins to the nuclear envelope and the centrosome.SUN蛋白定位于核膜和中心体所涉及结构的特征分析。
DNA Cell Biol. 2006 Oct;25(10):554-62. doi: 10.1089/dna.2006.25.554.