Jönsson U, Fägerstam L, Ivarsson B, Johnsson B, Karlsson R, Lundh K, Löfås S, Persson B, Roos H, Rönnberg I
Pharmacia Biosensor AB, Uppsala, Sweden.
Biotechniques. 1991 Nov;11(5):620-7.
We report here the development and application of a biosensor-based technology that employs surface plasmon resonance for label-free studies of molecular interactions in real time. The sensor chip interface, comprising a thin layer of gold deposited on a glass support, is derivatized with a flexible hydrophilic polymer to facilitate the attachment of specific ligands to the surface and to increase the dynamic range for surface concentration measurements. The sensor can be used to measure surface concentrations down to 10 pg/mm2. Typical coefficients of variation are from two to five percent. We anticipate that the ability to monitor multi-molecular complexes as they form will greatly contribute to the understanding of biorecognition and the structural basis of molecular function.
我们在此报告一种基于生物传感器的技术的开发与应用，该技术利用表面等离子体共振实时对分子相互作用进行无标记研究。传感器芯片界面由沉积在玻璃载体上的一薄层金组成，用柔性亲水性聚合物进行衍生化处理，以促进特定配体附着于表面，并增加表面浓度测量的动态范围。该传感器可用于测量低至10 pg/mm2的表面浓度。典型的变异系数为2%至5%。我们预计，在多分子复合物形成时对其进行监测的能力将极大地有助于理解生物识别及分子功能的结构基础。
