Bharucha Nikë, Kumar Anuj
Department of Molecular, Cellular, and Developmental Biology and the Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109-2216, USA.
Comb Chem High Throughput Screen. 2007 Sep;10(8):618-34. doi: 10.2174/138620707782507340.
The budding yeast Saccharomyces cerevisiae is well recognized as a preferred eukaryote for the development of genomic technologies and approaches. Accordingly, a sizeable complement of genomic resources has been developed in yeast, and this genomic foundation is now informing a wide variety of disciplines. In particular, yeast genomic methodologies are gaining an expanding foothold in drug development studies, most notably as a preliminary tool towards drug target identification. In this review, we highlight many applications of yeast genomics in the identification of targeted genes and pathways of small molecules or therapeutic drugs. The applicability of genome-wide resources of yeast disruption and deletion mutants for drug-sensitivity/resistance screening is presented here, along with a summary of microarray technologies for drug-based transcriptional profiling and synthetic interaction mapping. Applications of protein-interaction traps for potential drug target identification are also considered. Collectively, this overview of yeast genomics emphasizes the growing intersection between high-throughput model organism biology and medicinal chemistry an intersection promising tangible advances for both academic and pharmaceutical fields alike.
出芽酵母酿酒酵母被公认为是开发基因组技术和方法的首选真核生物。因此，酵母中已开发出大量的基因组资源，而这一基因组基础如今正为众多学科提供信息。特别是，酵母基因组方法在药物开发研究中所占的比重越来越大，最显著的是作为识别药物靶点的初步工具。在本综述中，我们重点介绍了酵母基因组学在识别小分子或治疗药物的靶向基因和途径方面的许多应用。本文介绍了酵母破坏和缺失突变体的全基因组资源在药物敏感性/抗性筛选中的适用性，以及用于基于药物的转录谱分析和合成相互作用图谱的微阵列技术总结。还考虑了蛋白质相互作用陷阱在潜在药物靶点识别中的应用。总体而言，对酵母基因组学的这一概述强调了高通量模式生物生物学与药物化学之间日益增加的交叉点——这一交叉点有望为学术和制药领域带来切实的进展。
