Smith Rosina E, Lespi Pablo, Di Luca María, Bustos Claudia, Marra Fernando A, de Alaniz María J T, Marra Carlos A
Hospital Penna, Bahía Blanca, Pcia. de Buenos Aires, Argentina.
Lipids. 2008 Jan;43(1):79-89. doi: 10.1007/s11745-007-3133-6. Epub 2007 Nov 29.
Antigen tumor markers employed in monitoring therapeutical approaches are limited by their specificity (Sp) and sensitivity (Se). The aim of this study was to investigate the suitability of a lipid tumor marker derived from ether-linked phospholipids and to compare it with others usually assayed in clinical practice. Complex lipids from normal and pathological breast, lung, and prostate tissue were isolated and analyzed by TLC and c-GLC methods. Results were compared as pooled samples, or by means of the averaged percent changes with respect to the composition observed in the normal tissue of the same patient. Sp, Se, negative-predictive (NPV) and positive- predictive values (PPV) were established for conventional markers and for the proposed lipid-derived marker. Results demonstrated that the content of monoenoic fatty acyl chains was significantly increased in total lipids, phosphatidylethanolamine, and especially in ethanolamine-containing ether lipids of neoplastic tissues with respect to their corresponding normal ones. Major changes were observed in the plasmalogen sub-fraction where the ratio monoenoic/saturated fatty acids can distinguish with high Se normal tissues from either benign or neoplastic tissues from breast, lung, or prostate lesions. Analyses of fatty acyl chains from ethanolamine-containing plasmalogens provided a reliable tumor marker that correlated with high Se and linearity with metastases spreading. This fact may be useful in prognosis of the most frequently observed human cancers.
用于监测治疗方法的抗原肿瘤标志物受其特异性（Sp）和敏感性（Se）的限制。本研究的目的是研究一种源自醚键连接磷脂的脂质肿瘤标志物的适用性，并将其与临床实践中常用检测的其他标志物进行比较。通过薄层层析（TLC）和毛细管气相色谱（c-GLC）方法对来自正常及病理状态下的乳腺、肺和前列腺组织的复合脂质进行分离和分析。结果作为合并样本进行比较，或通过相对于同一患者正常组织中观察到的组成的平均百分比变化进行比较。为传统标志物和所提议的脂质衍生标志物确定了Sp、Se、阴性预测值（NPV）和阳性预测值（PPV）。结果表明，与相应正常组织相比，肿瘤组织的总脂质、磷脂酰乙醇胺，尤其是含乙醇胺的醚脂中的单烯脂肪酸酰基链含量显著增加。在缩醛磷脂亚组分中观察到主要变化，其中单烯/饱和脂肪酸的比例可以以高敏感性区分正常组织与乳腺、肺或前列腺病变的良性或肿瘤组织。对含乙醇胺缩醛磷脂的脂肪酸酰基链分析提供了一种可靠的肿瘤标志物，其与高敏感性以及转移扩散的线性相关。这一事实可能有助于对最常见的人类癌症进行预后评估。
