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光对嗜热毁丝霉的影响以及ENVOY在此过程中的多种细胞作用。

Impact of light on Hypocrea jecorina and the multiple cellular roles of ENVOY in this process.

作者信息

Schuster Andrè, Kubicek Christian P, Friedl Martina A, Druzhinina Irina S, Schmoll Monika

机构信息

Division of Gene Technology and Applied Biochemistry, Institute for Chemical Engineering, Vienna University of Technology, Getreidemarkt 9/1665, A-1060 Wien, Austria.

出版信息

BMC Genomics. 2007 Dec 4;8:449. doi: 10.1186/1471-2164-8-449.

DOI:10.1186/1471-2164-8-449
PMID:18053205
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2234433/
Abstract

BACKGROUND

In fungi, light is primarily known to influence general morphogenesis and both sexual and asexual sporulation. In order to expand the knowledge on the effect of light in fungi and to determine the role of the light regulatory protein ENVOY in the implementation of this effect, we performed a global screen for genes, which are specifically effected by light in the fungus Hypocrea jecorina (anamorph Trichoderma reesei) using Rapid Subtraction Hybridization (RaSH). Based on these data, we analyzed whether these genes are influenced by ENVOY and if overexpression of ENVOY in darkness would be sufficient to execute its function.

RESULTS

The cellular functions of the detected light responsive genes comprised a variety of roles in transcription, translation, signal transduction, metabolism, and transport. Their response to light with respect to the involvement of ENVOY could be classified as follows: (i) ENVOY-mediated upregulation by light; (ii) ENVOY-independent upregulation by light; (iii) ENVOY-antagonized upregulation by light; ENVOY-dependent repression by light; (iv) ENVOY-independent repression by light; and (v) both positive and negative regulation by ENVOY of genes not responsive to light in the wild-type. ENVOY was found to be crucial for normal growth in light on various carbon sources and is not able to execute its regulatory function if overexpressed in the darkness.

CONCLUSION

The different responses indicate that light impacts fungi like H. jecorina at several cellular processes, and that it has both positive and negative effects. The data also emphasize that ENVOY has an apparently more widespread cellular role in this process than only in modulating the response to light.

摘要

背景

在真菌中，光主要影响一般形态发生以及有性和无性孢子形成。为了拓展对光在真菌中作用的认识，并确定光调节蛋白ENVOY在这种作用的实施过程中的作用，我们利用快速减法杂交（RaSH）对在嗜热毁丝霉（无性型里氏木霉）中受光特异性影响的基因进行了全基因组筛选。基于这些数据，我们分析了这些基因是否受ENVOY影响，以及在黑暗中过表达ENVOY是否足以执行其功能。

结果

检测到的光响应基因的细胞功能在转录、翻译、信号转导、代谢和运输中发挥多种作用。它们对光的响应以及ENVOY的参与情况可分类如下：（i）光介导的ENVOY上调；（ii）光介导的ENVOY非依赖性上调；（iii）光介导的ENVOY拮抗上调；光介导的ENVOY依赖性抑制；（iv）光介导的ENVOY非依赖性抑制；以及（v）ENVOY对野生型中对光无响应的基因的正负调控。发现ENVOY对于在各种碳源上的光照条件下正常生长至关重要，并且如果在黑暗中过表达则无法执行其调节功能。

结论

不同的响应表明光在多个细胞过程中影响嗜热毁丝霉这样的真菌，并且具有正负两方面的作用。数据还强调，ENVOY在这个过程中显然具有比仅调节对光的响应更广泛的细胞作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/831d/2234433/886cdb5fae09/1471-2164-8-449-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/831d/2234433/875758a68e5d/1471-2164-8-449-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/831d/2234433/ff385e061cac/1471-2164-8-449-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/831d/2234433/5eedd6dd74ac/1471-2164-8-449-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/831d/2234433/385776950e8c/1471-2164-8-449-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/831d/2234433/06e4c32ebf32/1471-2164-8-449-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/831d/2234433/886cdb5fae09/1471-2164-8-449-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/831d/2234433/875758a68e5d/1471-2164-8-449-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/831d/2234433/ff385e061cac/1471-2164-8-449-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/831d/2234433/5eedd6dd74ac/1471-2164-8-449-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/831d/2234433/385776950e8c/1471-2164-8-449-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/831d/2234433/06e4c32ebf32/1471-2164-8-449-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/831d/2234433/886cdb5fae09/1471-2164-8-449-6.jpg

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