枯草芽孢杆菌硫胺素补救酶TenA的诱变研究。
Mutagenesis studies on TenA: a thiamin salvage enzyme from Bacillus subtilis.
作者信息
Jenkins Amy L, Zhang Yang, Ealick Steven E, Begley Tadhg P
机构信息
Department of Chemistry and Chemical Biology, Cornell University, Ithaca, NY 14853, USA.
出版信息
Bioorg Chem. 2008 Feb;36(1):29-32. doi: 10.1016/j.bioorg.2007.10.005. Epub 2007 Dec 3.
Abstract
TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here, we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each of the active site residues are identified and a mechanism for the reaction is described.
摘要
TenA催化4-氨基-5-氨甲基-2-甲基嘧啶的水解反应，并参与碱基降解型硫胺素的补救合成。在此，我们描述了在与底物类似物及产物形成复合物的酶结构指导下对TenA活性位点进行的诱变。确定了每个活性位点残基的催化作用，并描述了该反应的机制。
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