Fine Daniel H, Markowitz Kenneth, Furgang David, Goldsmith Deborah, Ricci-Nittel Danette, Charles Christine H, Peng Penny, Lynch Michael C
Department of Oral Biology, New Jersey Dental School/University of Medicine and Dentistry of New Jersey, Newark, NJ 07103, USA.
J Periodontol. 2007 Oct;78(10):1935-42. doi: 10.1902/jop.2007.070120.
Studies have shown that the nature and amount of supragingival plaque can influence the composition of the contiguous subgingival flora. The purpose of this study was to investigate the effect of rinsing with an essential oil antimicrobial mouthrinse on levels of representative subgingival bacteria in subjects with mild to moderate periodontitis.
This controlled study used a randomized, double-masked, 2 x 2 crossover design. After baseline subgingival sampling, 37 qualifying subjects rinsed with the essential oil mouthrinse or a negative control twice daily for 14 days, with a post-treatment sample obtained on day 15. Following a washout period, the procedure was repeated with the alternative rinse. Target organisms enumerated were Porphyromonas gingivalis, Fusobacterium nucleatum, Veillonella sp., and total anaerobes. Intergroup comparisons of post-treatment log-transformed colony forming unit counts were made using analysis of covariance.
After 14 days of twice-daily rinsing, the level of each of the target subgingival organisms was significantly lower in the essential oil group than in the control group (P < 0.001), with percent reductions ranging from 66.3% to 79.2%.
Rinsing with the essential oil antiplaque/antigingivitis mouthrinse can have significant antimicrobial activity against subgingival periodontopathogens. Most likely, the antimicrobial effect was mediated primarily by disruption of the contiguous supragingival plaque by the mouthrinse. Because studies have shown that control of supragingival plaque can influence the onset and/or progression of periodontitis, additional studies on non-prescription antimicrobial oral care products may lead to new regimens for decreasing the burden of periodontal diseases in the population.
研究表明，龈上菌斑的性质和数量会影响相邻龈下菌群的组成。本研究的目的是调查使用含精油抗菌漱口水漱口对轻度至中度牙周炎患者龈下代表性细菌水平的影响。
本对照研究采用随机、双盲、2×2交叉设计。在进行基线龈下采样后，37名符合条件的受试者每天用含精油漱口水或阴性对照漱口水漱口两次，持续14天，并在第15天采集治疗后样本。经过洗脱期后，用另一种漱口水重复该过程。所列举的目标微生物为牙龈卟啉单胞菌、具核梭杆菌、韦荣氏菌属和总厌氧菌。使用协方差分析对治疗后对数转换的菌落形成单位计数进行组间比较。
每天漱口两次，持续14天后，精油组中每种目标龈下微生物的水平均显著低于对照组（P < 0.001），减少百分比范围为66.3%至79.2%。
使用含精油防菌斑/防牙龈炎漱口水漱口可对龈下牙周病原体产生显著的抗菌活性。抗菌作用很可能主要是由漱口水对相邻龈上菌斑的破坏介导的。由于研究表明控制龈上菌斑可影响牙周炎的发生和/或进展，对非处方抗菌口腔护理产品的进一步研究可能会带来减轻人群牙周疾病负担的新方案。
