一种用于硫醇生物成像的高选择性荧光探针。
A highly selective fluorescent probe for thiol bioimaging.
作者信息
Bouffard Jean, Kim Youngmi, Swager Timothy M, Weissleder Ralph, Hilderbrand Scott A
机构信息
Department of Chemistry, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
出版信息
Org Lett. 2008 Jan 3;10(1):37-40. doi: 10.1021/ol702539v. Epub 2007 Dec 7.
A new fluorescent turn-on probe (3) for the selective sensing and bioimaging of thiols is reported. In aqueous buffer solutions at physiological pH, thiols cleave the 2,4-dinitrobenzenesulfonyl group to release the red-emissive donor-acceptor fluorophore (4). The probe displays excellent immunity to interference from nitrogen and oxygen nucleophiles and the imaging of thiols in living cells is demonstrated.
报道了一种用于硫醇选择性传感和生物成像的新型荧光开启探针（3）。在生理pH值的水性缓冲溶液中，硫醇会裂解2,4-二硝基苯磺酰基，释放出发射红色荧光的供体-受体荧光团（4）。该探针对氮和氧亲核试剂的干扰具有出色的抗性，并证明了其对活细胞中硫醇的成像能力。
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