Bizzarri Anna Rita, Cannistraro Salvatore
Biophysics and Nanoscience Centre, CNISM, Facolta' di Scienze, Università della Tuscia, Viterbo, Italy.
Nanomedicine. 2007 Dec;3(4):306-10. doi: 10.1016/j.nano.2007.09.005.
We present a method based on surface-enhanced Raman spectroscopy and exploiting a protein-protein recognition process able to detect thrombin at subpicomolar concentrations. Gold nanoparticles (NPs) were capped with a bifunctional molecule capable of forming a covalent link with the aromatic residues of the protein moiety. The typical vibrations of the diazo bond established between the bifunctional molecule and the target protein are found to be selectively enhanced by the conjugated gold NPs, and therefore constitutes the Raman marker. After the interaction of functionalized NPs with antithrombin as a sensitive recognition element, immobilized on a capture substrate, we have detected thrombin at a concentration of about 10(-13) M.
我们提出了一种基于表面增强拉曼光谱并利用蛋白质-蛋白质识别过程的方法，该方法能够检测亚皮摩尔浓度的凝血酶。金纳米颗粒（NPs）用一种双功能分子包覆，该分子能够与蛋白质部分的芳香族残基形成共价键。发现双功能分子与靶蛋白之间形成的重氮键的典型振动被共轭金纳米颗粒选择性增强，因此构成拉曼标记物。在功能化纳米颗粒与作为敏感识别元件的抗凝血酶相互作用后，将其固定在捕获基质上，我们检测到浓度约为10^(-13) M的凝血酶。
