Cheng Anwei, Wan Fachun, Wang Jiaqi, Jin Zhengyu, Xu Xueming
School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Int Immunopharmacol. 2008 Jan;8(1):43-50. doi: 10.1016/j.intimp.2007.10.006. Epub 2007 Oct 29.
The objective of this study was to evaluate the immunomodulatory effects of the purified glycyrrhiza polysaccharides (GP) on the activity of macrophages. A purified fraction of water-soluble polysaccharides, with estimated molecular weight of 10 kDa, was isolated from Glycyrrhiza uralensis Fish using ion exchange and size exclusion chromatography. The results indicate that GP increased the pinocytic activity, the production of nitric oxide (NO), interleukin-1 (IL-1), IL-6 and IL-12 in a dose-dependent manner. The production of IL-1 was induced by GP at a dose of 10 microg/mL; but, NO, IL-6 and IL-12 was significantly induced at 100 microg/mL. A time-dependent enhancement showed that the production of IL-1, NO and IL-12 were significantly increased within 6 h. Superoxide anion (O(2)(-)) production by macrophages from GP-treated mice was higher than that of cells from untreated mice. Moreover, cells from both untreated and treated mice responded to phorbol 12-myristate 13-acetate (PMA) treatment; however, the O(2)(-) production was higher in the cells from treated mice than that of cells from untreated mice. Our data suggest that the beneficial therapeutic effects of GP may be attributed partly to its ability to modulate macrophage immune functions.
本研究的目的是评估纯化的甘草多糖（GP）对巨噬细胞活性的免疫调节作用。使用离子交换和尺寸排阻色谱法从乌拉尔甘草中分离出一种纯化的水溶性多糖级分，其估计分子量为10 kDa。结果表明，GP以剂量依赖的方式增加了巨噬细胞的吞噬活性、一氧化氮（NO）、白细胞介素-1（IL-1）、IL-6和IL-12的产生。GP在剂量为10μg/mL时诱导IL-1的产生；但在100μg/mL时，NO、IL-6和IL-12被显著诱导。时间依赖性增强表明，IL-1、NO和IL-12的产生在6小时内显著增加。来自GP处理小鼠的巨噬细胞产生的超氧阴离子（O(2)(-)）高于未处理小鼠的细胞。此外，未处理和处理小鼠的细胞对佛波醇12-肉豆蔻酸酯13-乙酸酯（PMA）处理均有反应；然而，处理小鼠的细胞中O(2)(-)的产生高于未处理小鼠的细胞。我们的数据表明，GP的有益治疗作用可能部分归因于其调节巨噬细胞免疫功能的能力。
