Doanh Pham Ngoc, Shinohara Akio, Horii Yoichiro, Habe Shigehisa, Nawa Yukifumi, Le Nguyen Thi
Department of Parasitology, Institute of Ecology and Biological Resources, Vietnamese Academy of Science and Technology, Hanoi, Vietnam.
Parasitol Res. 2008 Mar;102(4):677-83. doi: 10.1007/s00436-007-0811-5. Epub 2007 Dec 11.
During an epidemiological survey for Paragonimus and paragonimosis in northern Vietnam, we found extremely large excysted metacercariae (2.50 +/- 0.14 mm in length and 0.72 +/- 0.08 mm in width; mean +/- standard deviation of 20 samples) in mountainous crabs, Potamiscus mieni. Adult worms were successfully obtained by intraperitoneal injection with those large excysted metacercariae in a cat. Morphological and morphometric data of those large excysted metacercariae and the adult worms derived from them are identical to those of Paragonimus proliferus found in Yunnan province, China. However, when second internal transcribed spacer region and cytochrome oxidase subunit 1 gene sequences of those metacercariae and adult worms were compared with those of known Paragonimus spp. deposited in the GenBank, they were almost completely identical to those of Paragonimus hokuoensis metacercariae in China, of which adult worms have never been reported. This is the first record of P. proliferus in Vietnam and the first record from outside of China. Phylogenetic relationship between P. proliferus and P. hokuoensis is discussed.
在对越南北部并殖吸虫和并殖吸虫病进行的一项流行病学调查中，我们在山蟹米氏溪蟹（Potamiscus mieni）体内发现了极大的脱囊后尾蚴（20个样本的长度为2.50±0.14毫米，宽度为0.72±0.08毫米；平均值±标准差）。通过给一只猫腹腔注射这些大型脱囊后尾蚴成功获得了成虫。这些大型脱囊后尾蚴及其衍生的成虫的形态学和形态测量数据与在中国云南省发现的多殖并殖吸虫（Paragonimus proliferus）相同。然而，当将这些尾蚴和成虫的第二内部转录间隔区及细胞色素氧化酶亚基1基因序列与GenBank中已知的并殖吸虫物种的序列进行比较时，它们几乎与中国福建并殖吸虫（Paragonimus hokuoensis）尾蚴的序列完全相同，而该虫的成虫从未有过报道。这是多殖并殖吸虫在越南的首次记录，也是在中国境外的首次记录。文中还讨论了多殖并殖吸虫与福建并殖吸虫之间的系统发育关系。
