Choi Wonjae, Kim Ji-Su, Lee Do-Hyun, Lee Kyung-Kwang, Koo Deog-Bon, Park Je-Kyun
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 335 Gwahangno, Yuseong-gu, Daejeon, 305-701, Republic of Korea.
Biomed Microdevices. 2008 Jun;10(3):337-45. doi: 10.1007/s10544-007-9141-8.
This paper reports a new dielectrophoretic separation method of porcine oocytes for in vitro fertilization. Conventional manual selection of oocyte highly depends on the expert's experience and lacks universal standards for identifying the quality of oocyte. In this study, an electrode array chip with castellated shape was developed to evaluate dielectrophoretic velocities of oocytes, under applied bias conditions with an AC 3 V waveform at 1 MHz for 15 s. Based on different dielectrophoresis (DEP) response, the selected group of oocytes that moved showed a better developmental potential than the group of oocytes that stayed, representing a higher rate of blastocyst formation and a lower rate of polyspermic fertilization. In addition, the overall developmental potential of oocytes selected by the DEP device was comparable to that of oocytes selected by conventional manual method. These results demonstrate that the difference in dielectrophoretic velocity can be used to establish an objective criterion for the selection of oocytes. Consequently, this method will open the possibility to develop an automatic tool for oocyte selection, which would be helpful for assisted reproductive technologies such as transgenic and clonal animal production.
本文报道了一种用于体外受精的猪卵母细胞介电泳分离新方法。传统的手动挑选卵母细胞高度依赖专家经验，且缺乏鉴定卵母细胞质量的通用标准。在本研究中，开发了一种带有锯齿形状的电极阵列芯片，用于在1 MHz的交流3 V波形、施加偏置条件下15 s的情况下评估卵母细胞的介电泳速度。基于不同的介电泳（DEP）响应，移动的所选卵母细胞组比停留的卵母细胞组显示出更好的发育潜力，表现为更高的囊胚形成率和更低的多精受精率。此外，通过DEP装置挑选的卵母细胞的总体发育潜力与通过传统手动方法挑选的卵母细胞相当。这些结果表明，介电泳速度的差异可用于建立卵母细胞选择的客观标准。因此，该方法将为开发卵母细胞选择的自动工具开辟可能性，这将有助于转基因和克隆动物生产等辅助生殖技术。
