Li Wen-Lan, Wang Yan-Ping, Ji Yu-Bin, Fang Yu-Qi, Zhao Pei
Institute of Materia Medica, Harbin University, Commerce Postdoctoral Programme, Harbin 150076, China.
Zhongguo Zhong Yao Za Zhi. 2007 Mar;32(5):396-400.
To study systematically the factors which affect separation and purification of the total alkaloids and mesaconitine with X -5 macroporous resin.
With the content of the total alkaloids and mesaconitine as parameters, the optimum condition of absorption and elution were studied in the process of the purification with X -5 macroporous resin.
The X - 5 macroporous resin yielded the best separating efficiency when the concentration of the extracted solution was 1 g raw material per 1 mL, pH 12.0, the absorptive time of 6 hour and the volume of 95% ethanol (7BV pH 8) as the eluant; X -5 macroporous resins was used five times in a reproducible way. The rate of extraction and content of the total alkaloid were 80% and 30% respectively after purification with X - 5 macroprous resin.
The method can increase the purity of mesaconitine and total alkaloids.
系统研究影响X-5大孔树脂分离纯化总生物碱和中乌头碱的因素。
以总生物碱和中乌头碱的含量为指标，研究X-5大孔树脂纯化过程中的最佳吸附和洗脱条件。
当提取液浓度为每1 mL含1 g原料、pH值为12.0、吸附时间为6小时、以95%乙醇（7BV，pH 8）为洗脱剂时，X-5大孔树脂的分离效率最佳；X-5大孔树脂可重复使用5次。用X-5大孔树脂纯化后，总生物碱的提取率和含量分别为80%和30%。
该方法可提高中乌头碱和总生物碱的纯度。
