Hanada Yasutaka, Sugioka Koji, Kawano Hiroyuki, Ishikawa Ikuko Shihira, Miyawaki Atsushi, Midorikawa Katsumi
RIKEN-The Institute of Physical and Chemical Research, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
Biomed Microdevices. 2008 Jun;10(3):403-10. doi: 10.1007/s10544-007-9149-0.
We demonstrate the fabrication of three-dimensional (3-D) hollow microstructures embedded in photostructurable glass by a nonlinear multiphoton absorption process using a femtosecond (fs) laser. Fs laser direct writing followed by annealing and successive wet etching in dilute hydrofluoric (HF) acid solution resulted in the rapid manufacturing of microchips with 3-D hollow microstructures for the dynamic observation of living microorganisms and cells in fresh water. The embedded microchannel structure enables us to analyze the continuous motion of Euglena gracilis. A microchamber with a movable microneedle demonstrates its ability for the elucidation of the information transmission process in Pleurosira laevis. Such microchips, referred to as nano-aquariums realize the efficient and highly functional observation of microorganisms and cells.
我们展示了通过飞秒（fs）激光的非线性多光子吸收过程，在可光结构化玻璃中制造三维（3-D）中空微结构。飞秒激光直接写入，随后进行退火，并在稀氢氟酸（HF）溶液中连续湿法蚀刻，从而快速制造出具有3-D中空微结构的微芯片，用于动态观察淡水中的活微生物和细胞。嵌入式微通道结构使我们能够分析纤细裸藻的连续运动。带有可移动微针的微腔展示了其阐明平滑双眉藻信息传递过程的能力。这种被称为纳米水族馆的微芯片实现了对微生物和细胞的高效且高功能观察。
