Takeshi K, Fujii N, Kimura K, Yamakawa K, Nakamura S, Kumagai M, Oguma K
Hokkaido Institute of Public Health, Japan.
Microbiol Immunol. 1991;35(12):1095-103. doi: 10.1111/j.1348-0421.1991.tb01631.x.
The germination of spores of a neurotoxigenic Clostridium butyricum strain (BL 6340), which was isolated from infant botulism in Italy, and that of a nontoxigenic C. butyricum type strain (NCIB 7423) were studied. The spores of BL 6340 strain were killed at 80 C for 10 min, and required the mixture of L-alanine, L-lactate, glucose and bicarbonate for their optimal germination. These characteristics are the same as those of Clostridium botulinum type E strain, but different from those of NCIB 7423 strain. In a hybridization test, however, the labeled DNAs extracted from NCIB 7423 strain highly (98%) hybridized to the DNAs of the BL 6340 strain, but little (45%) to the DNAs of C. botulinum type E strain. The biochemical properties of the BL 6340 and NCIB 7423 strains were identical, but different from those of C. botulinum type E. These data confirmed that the BL 6340 strain belongs to C. butyricum species, but that only its characteristics of toxin production, its minimum requirements for germination, and the behavior of its spores to heat treatment are the same as those of C. botulinum type E. When conventionally raised suckling mice were injected with 5 x 10(7) spores of BL 6340 strain intra- or orogastrically, botulism was not observed. However, 8- to 13-day-old mice had type E botulinum toxin in the large intestine 3 days after introduction of its spores.
对从意大利婴儿肉毒中毒病例中分离出的产神经毒素丁酸梭菌菌株（BL 6340）以及非产毒丁酸梭菌模式菌株（NCIB 7423）的孢子萌发情况进行了研究。BL 6340菌株的孢子在80℃下加热10分钟可被杀死，其最佳萌发需要L-丙氨酸、L-乳酸、葡萄糖和碳酸氢盐的混合物。这些特性与E型肉毒梭菌菌株相同，但与NCIB 7423菌株不同。然而，在杂交试验中，从NCIB 7423菌株提取的标记DNA与BL 6340菌株的DNA高度杂交（98%），但与E型肉毒梭菌菌株的DNA杂交程度较低（45%）。BL 6340和NCIB 7423菌株的生化特性相同，但与E型肉毒梭菌不同。这些数据证实，BL 6340菌株属于丁酸梭菌属，但只有其毒素产生特性、萌发的最低要求以及孢子对热处理的反应与E型肉毒梭菌相同。当给常规饲养的乳鼠经腹腔或经口注射5×10⁷个BL 6340菌株的孢子时，未观察到肉毒中毒现象。然而，8至13日龄的小鼠在接种其孢子3天后，大肠中出现了E型肉毒毒素。
