Meng X, Yamakawa K, Zou K, Wang X, Kuang X, Lu C, Wang C, Karasawa T, Nakamura S
Department of Bacteriology, School of Medicine, Kanazawa University, Kanazawa 920-8640, Japan.
J Med Microbiol. 1999 Feb;48(2):133-137. doi: 10.1099/00222615-48-2-133.
Soil specimens collected from a site around the home of patients with food-borne type E. botulism probably caused by neurotoxigenic Clostridium butyricum in Guanyun, Jiangsu province, China, were examined for the presence of neurotoxigenic C. butyricum. Five lakeside sites of Weishan lake, in an area near to the sites where the type E. botulism outbreaks caused by neurotoxigenic C. butyricum occurred were also surveyed. Type E toxin-producing C. butyricum was isolated from soil from four sites including the site in Guanyun. Polymerase chain reaction assay demonstrated the presence of the type E toxin gene in all the toxigenic isolates. The biochemical properties of the isolates from the Guanyun soil and the lakeside soil were identical except for inulin fermentation and starch hydrolysis properties. These results indicate that neurotoxigenic C. butyricum has its principal habitat in soil.
从中国江苏省灌云县一起可能由产神经毒素丁酸梭菌引起的食源性E型肉毒中毒患者家中周围地点采集的土壤标本，被检测是否存在产神经毒素丁酸梭菌。还对微山湖五个湖边地点进行了调查，这些地点靠近由产神经毒素丁酸梭菌引起E型肉毒中毒暴发的地点。从包括灌云县地点在内的四个地点的土壤中分离出产E毒素的丁酸梭菌。聚合酶链反应分析表明，所有产毒分离株中都存在E毒素基因。除菊糖发酵和淀粉水解特性外，灌云县土壤和湖边土壤分离株的生化特性相同。这些结果表明，产神经毒素丁酸梭菌主要生境在土壤中。
