Uludağ Yildiz, Li Xin, Coleman Heather, Efstathiou Stacey, Cooper Matthew A
Akubio Ltd, 181 Cambridge Science Park, Cambridge, UK CB4 0GJ.
Analyst. 2008 Jan;133(1):52-7. doi: 10.1039/b711850c. Epub 2007 Sep 21.
We describe the detection of specific, conserved DNA sequences of herpes simplex virus (HSV) type 1 by means of a novel, high sensitivity acoustic biosensor. Repeated assays on planar and polymeric carboxylic acid- and biotin-presenting surface chemistries enabled statistical comparison of assay specificity and sensitivity and evaluation of assay Z-factor scores. Using a three minute hybridisation with NeutrAvidin capture for signal enhancement, it was possible to detect HSV viral nucleic acids at 5.2 x 10(-11) M concentration.
我们描述了通过一种新型的高灵敏度声学生物传感器检测单纯疱疹病毒1型（HSV）的特定保守DNA序列。在平面和聚合物羧酸及生物素呈现表面化学上进行重复测定，能够对测定的特异性和灵敏度进行统计学比较，并评估测定的Z因子得分。通过与中性抗生物素蛋白捕获进行三分钟杂交以增强信号，能够检测浓度为5.2×10^(-11) M的HSV病毒核酸。
