Wilkins E E, Howell P I, Benedict M Q
Centers for Disease Control and Prevention (CDC) and Atlanta Research and Education Foundation, Atlanta, GA, USA.
Insect Mol Biol. 2007 Dec;16(6):735-41. doi: 10.1111/j.1365-2583.2007.00769.x.
We observed Anopheles gambiae sensu stricto stocks that contained both Mopti (M) and Savanna (S) rDNA intergenic spacers (IGS). ASEMBO1 male IGS sequences consistently had a mixture. A diagnostic M and S Hha I restriction enzyme site in these fragments was concordant with two SNPs associated with M and S. Standard M and S stocks demonstrated X-chromosome-only inheritance of the rDNA form, but the ASEMBO1 males showed X and Y chromosome linkage of mixed rDNA. The metaphase Y chromosomes of ASEMBO1 contained a significantly larger amount of DNA relative to the X than a standard S stock. Analysis of wild A. gambiae males from the putative location of origin of the ASEMBO1 stock did not detect the same pattern of polymorphism observed in the laboratory stock but did detect heterogeneous arrays including some missing a diagnostic Hha I restriction site. These results demonstrate that M and S IGS types can occur within the rDNA arrays of a chromatid in laboratory A. gambiae stocks, and some A. gambiae s.s. have rDNA on the Y chromosome.
我们观察到冈比亚按蚊指名亚种种群同时包含莫普提（M）和萨凡纳（S）核糖体DNA基因间隔区（IGS）。ASEMBO1雄性的IGS序列始终是混合的。这些片段中诊断性的M和S Hha I限制性酶切位点与两个分别与M和S相关的单核苷酸多态性一致。标准的M和S种群显示核糖体DNA形式仅通过X染色体遗传，但ASEMBO1雄性显示混合核糖体DNA的X和Y染色体连锁。相对于标准S种群，ASEMBO1的中期Y染色体含有显著更多的DNA。对来自ASEMBO1种群假定起源地的野生冈比亚按蚊雄性的分析未检测到在实验室种群中观察到的相同多态性模式，但确实检测到了异质阵列，包括一些缺少诊断性Hha I限制性酶切位点的阵列。这些结果表明，M和S IGS类型可出现在实验室冈比亚按蚊种群染色单体的核糖体DNA阵列中，并且一些冈比亚按蚊指名亚种在Y染色体上有核糖体DNA。
