Boulesteix M, Simard F, Antonio-Nkondjio C, Awono-Ambene H P, Fontenille D, Biémont C
Laboratoire de Biométrie et Biologie Evolutive, UMR 5558, CNRS, Université Claude Bernard Lyon1, 69622 Villeurbanne Cedex, France.
Mol Ecol. 2007 Jan;16(2):441-52. doi: 10.1111/j.1365-294X.2006.03150.x.
The insertion polymorphism of five transposable element (TE) families was studied by Southern blots in several populations of the M and S molecular forms of the mosquito Anopheles gambiae sensu stricto from southern Cameroon. We showed that the mean TE insertion site number and the within-population insertion site polymorphism globally differed between the M and S molecular forms. The comparison of the TE insertion profiles of the populations revealed a significant differentiation between these two molecular forms (0.163 < Phi(ST) < 0.371). We cloned several insertions of a non-LTR retrotransposon (Aara8) that were fixed in one form and absent in the other one. The only insertion that could be clearly located on a chromosome arm mapped to cytological division 6 of chromosome X, confirming the importance of this region in the ongoing speciation between the M and S molecular forms.
通过Southern杂交技术，在喀麦隆南部冈比亚按蚊狭义种的M和S分子型的几个群体中，研究了五个转座元件（TE）家族的插入多态性。我们发现，M和S分子型之间，平均TE插入位点数量和群体内插入位点多态性总体上存在差异。群体的TE插入图谱比较显示，这两种分子型之间存在显著分化（0.163 < Phi(ST) < 0.371）。我们克隆了一个非LTR反转录转座子（Aara8）的几个插入片段，这些插入片段在一种分子型中固定存在，而在另一种分子型中缺失。唯一能明确定位在染色体臂上的插入片段，定位于X染色体的细胞学分区6，证实了该区域在M和S分子型正在进行的物种形成中的重要性。
