Gottfredsson M, Erlendsdottir H, Gudmundsson S
Department of Internal Medicine, Borgarspitalinn, Reykjavik, Iceland.
Antimicrob Agents Chemother. 1991 Dec;35(12):2658-61. doi: 10.1128/AAC.35.12.2658.
The duration of the postantibiotic effect (PAE) determined by bacterial CO2 production measured by using the BACTEC NR 730 blood culture system was compared with PAEs determined by standard viability counting. PAEs for Staphylococcus aureus after exposure to dicloxacillin, vancomycin, rifampin, gentamicin, and ciprofloxacin and for Escherichia coli after exposure to ampicillin, gentamicin, and ciprofloxacin were quantitated by the two methods, and an excellent correlation (r = 0.93) was demonstrated. The difference in the PAE durations determined by the two methods was 0.1 +/- 0.4 (mean +/- standard deviation) h. Thus, the BACTEC CO2 generation method provides a simple, alternate way of determining the PAE in vitro.
采用BACTEC NR 730血培养系统通过测量细菌二氧化碳生成量所确定的抗生素后效应（PAE）持续时间，与通过标准活菌计数法确定的PAE进行了比较。用这两种方法对金黄色葡萄球菌在接触双氯西林、万古霉素、利福平、庆大霉素和环丙沙星后以及大肠杆菌在接触氨苄西林、庆大霉素和环丙沙星后的PAE进行了定量，结果显示两者具有良好的相关性（r = 0.93）。两种方法所确定的PAE持续时间差异为0.1±0.4（平均值±标准差）小时。因此，BACTEC二氧化碳生成法为体外确定PAE提供了一种简单的替代方法。
