I型单纯疱疹病毒密切相关毒株DNA分子大小的变异

Variation in the molecular size of the DNA from closely related strains of type I herpes simplex virus.

作者信息

Wagner E K, Swanstrom R I, Rice M, Howell L, Lane J

出版信息

Biochim Biophys Acta. 1976 Jun 18;435(2):192-205. doi: 10.1016/0005-2787(76)90250-1.

Abstract

We have investigated the comparative genome size of five strains of HSV type 1 (HSV-1). Two of these strains have a common origin and differ in their plaque morphology on HeLa cells, one strain is a wild type isolate, and two others are established laboratory strains. All strains show high sequence homology based on the melting behavior of heteroduplexes. The greatest sequence divergence between any two strains was found to be no more than 10%. There are real differences in the size of the genomes of these strains of HSV-1 as measured by electron microscopy. The shortest and largest genomes measured differ in size by 10%, however, the size of the genomes of all strains are within 5% of a median valve of 87-88X106.

摘要

我们研究了五株1型单纯疱疹病毒（HSV-1）的比较基因组大小。其中两株有共同起源，在HeLa细胞上的噬斑形态不同，一株是野生型分离株，另外两株是已建立的实验室菌株。基于异源双链体的解链行为，所有菌株都显示出高度的序列同源性。发现任意两株之间的最大序列差异不超过10%。通过电子显微镜测量，这些HSV-1菌株的基因组大小存在实际差异。测得的最短和最长基因组大小相差10%，然而，所有菌株的基因组大小都在87 - 88×10⁶中位值的5%范围内。