Uddman R, Luts A, Absood A, Arimura A, Ekelund M, Desai H, Håkanson R, Hambreaus G, Sundler F
Department of Otorhinolaryngology, General Hospital, Malmö, Sweden.
Regul Pept. 1991 Nov 26;36(3):415-22. doi: 10.1016/0167-0115(91)90074-q.
The lower esophagus of guinea-pig, cat, sheep and man was analyzed for pituitary adenylate cyclase activating peptide (PACAP), a novel vasoactive intestinal peptide (VIP)-like peptide, using immunocytochemistry and radioimmunoassay. PACAP-immunoreactive nerve fibers were numerous in the longitudinal and circular muscle layers of sheep and man, moderate in numbers in cat, while being few in the esophagus of guinea-pig. A few PACAP-immunoreactive nerve cell bodies and numerous nerve fibers were seen in the myenteric ganglia of the esophagus of cat, sheep and man. In the lower esophagus of cat, sheep and man all PACAP-containing nerve cell bodies and nerve fibers stored VIP. The results of radioimmunoassay of PACAP in extracts of specimens from man were in good agreement with the immunocytochemical findings. High performance liquid chromatography revealed one major peak of PACAP-like immunoreactivity in extracts of human esophagus. We suggest that neuronal PACAP may serve to modulate motor activity and secretion in the lower esophageal sphincter region.
利用免疫细胞化学和放射免疫分析法，对豚鼠、猫、绵羊和人类的食管下段进行了分析，以检测垂体腺苷酸环化酶激活肽（PACAP），一种新型的血管活性肠肽（VIP）样肽。PACAP免疫反应性神经纤维在绵羊和人类的纵行和环行肌层中数量众多，在猫中数量适中，而在豚鼠食管中则很少。在猫、绵羊和人类食管的肌间神经节中可见少数PACAP免疫反应性神经细胞体和众多神经纤维。在猫、绵羊和人类的食管下段，所有含PACAP的神经细胞体和神经纤维都储存有VIP。对人类标本提取物中PACAP的放射免疫分析结果与免疫细胞化学结果高度一致。高效液相色谱法显示，人类食管提取物中有一个主要的PACAP样免疫反应性峰。我们认为，神经元PACAP可能有助于调节食管下括约肌区域的运动活动和分泌。
