Elsås T, Uddman R, Sundler F
Department of Ophthalmology, University Hospital of Trondheim, Norway.
Graefes Arch Clin Exp Ophthalmol. 1996 Sep;234(9):573-80. doi: 10.1007/BF00448802.
To study the occurrence and distribution of the neuropeptide pituitary adenylate cyclase-activating peptide (PACAP) in ocular and orbital structures of the cat.
Immunocyto-chemistry to localize PACAP and double immunostaining to detect co-localization of PACAP with other neuropeptides.
Numerous PACAP-immunoreactive nerve fibers were observed in the lacrimal gland, choroid and retroocular arteries. There was a sparse supply of PACAP-containing nerve fibers in the iris, ciliary body and conjunctiva. Subpopulations of PACAP-containing nerve fibers stored vasoactive intestinal peptide (VIP) or calcitonin gene-related peptide (CGRP). Around 10% of the ganglion cells in the sphenopalatine ganglion harbored PACAP immunoreactivity. In the trigeminal ganglion around 5% of the neuronal cell bodies and in the ciliary ganglion only occasional ganglion cells contained PACAP immunoreactivity. PACAP immunoreactivity co-localized with VIP in the sphenopalatine ganglion and with CGRP in the trigeminal ganglion.
PACAP-containing nerves in the eye and associated structures demonstrate a distribution pattern resembling that of VIP. Subpopulations of nerve fibers containing PACAP immunoreactivity store VIP or CGRP immunoreactivity. Neuronal PACAP in the eye and orbit may take part in regulation of smooth muscle tone, glandular secretion and sensory processing.
研究神经肽垂体腺苷酸环化酶激活肽（PACAP）在猫眼和眼眶结构中的发生及分布情况。
采用免疫细胞化学方法定位PACAP，并通过双重免疫染色检测PACAP与其他神经肽的共定位情况。
在泪腺、脉络膜和眼后动脉中观察到大量PACAP免疫反应性神经纤维。虹膜、睫状体和结膜中含PACAP的神经纤维供应稀少。含PACAP的神经纤维亚群储存血管活性肠肽（VIP）或降钙素基因相关肽（CGRP）。蝶腭神经节中约10%的神经节细胞具有PACAP免疫反应性。在三叉神经节中，约5%的神经元细胞体以及在睫状神经节中仅有偶尔的神经节细胞含有PACAP免疫反应性。PACAP免疫反应性在蝶腭神经节中与VIP共定位，在三叉神经节中与CGRP共定位。
眼及相关结构中含PACAP的神经呈现出与VIP相似的分布模式。含PACAP免疫反应性的神经纤维亚群储存VIP或CGRP免疫反应性。眼和眼眶中的神经元PACAP可能参与平滑肌张力、腺体分泌和感觉处理的调节。
