体外循环与非体外循环冠状动脉搭桥术患者体内的热休克蛋白27、60、70、90α及20S蛋白酶体
Heat shock proteins 27, 60, 70, 90alpha, and 20S proteasome in on-pump versus off-pump coronary artery bypass graft patients.
作者信息
Szerafin Tamas, Hoetzenecker Konrad, Hacker Stefan, Horvath Ambrus, Pollreisz Andreas, Arpád Péterffy, Mangold Andreas, Wliszczak Tina, Dworschak Martin, Seitelberger Rainald, Wolner Ernst, Ankersmit Hendrik J
机构信息
Department of Cardiac Surgery, University of Debrecen, Debrecen, Hungary.
出版信息
Ann Thorac Surg. 2008 Jan;85(1):80-7. doi: 10.1016/j.athoracsur.2007.06.049.
BACKGROUND
The secretion of heat shock protein (HSP) 27, HSP60, HSP70, HSP90alpha, 20S proteasome, and their correlations to proinflammatory cytokine interleukin-6 is unknown in patients undergoing on-pump versus off-pump coronary artery bypass graft (CABG) operation.
METHODS
Forty patients were included in this explorative study (on- versus off-pump CABG, each n = 20). Serum samples were obtained before and 30 minutes, 60 minutes, and 24 hours after CABG operation. Enzyme-linked immunosorbent assay technique was utilized to determine soluble HSP27, 60, 70, and 90alpha, 20S proteasome, and levels of interleukin-6.
RESULTS
Serum levels of HSP are increased in patients undergoing on-pump CABG operation as compared with off-pump CABG technique. These differences were highly significant for HSP27, 70, and 90alpha at 60 minutes after initiation of cardiopulmonary bypass (all, p < 0.001). Concentrations of soluble 20S proteasome were increased 24 hours after operation in on- and off-pump CABG patients (p < 0.001) and correlated significantly with the serum content of HSP 27, 70, and 90alpha at 60 minutes after initiation of cardiopulmonary bypass (p < 0.001). No correlation was found when comparing interleukin-6 levels with intravascular leakage of HSP and 20S proteasome after CABG operation.
CONCLUSIONS
We conclude from our data that the innate immune system is activated owing to spillage of known immune modulatory and apoptosis-associated proteins after CABG operation.
背景
在接受体外循环与非体外循环冠状动脉旁路移植术（CABG）的患者中，热休克蛋白（HSP）27、HSP60、HSP70、HSP90α、20S蛋白酶体的分泌及其与促炎细胞因子白细胞介素-6的相关性尚不清楚。
方法
本探索性研究纳入40例患者（体外循环与非体外循环CABG各20例）。在CABG手术前、术后30分钟、60分钟和24小时采集血清样本。采用酶联免疫吸附测定技术测定可溶性HSP27、60、70和90α、20S蛋白酶体以及白细胞介素-6水平。
结果
与非体外循环CABG技术相比，接受体外循环CABG手术的患者血清HSP水平升高。在体外循环开始60分钟时，HSP27、70和90α的这些差异非常显著（均p<0.001）。体外循环与非体外循环CABG患者术后24小时可溶性20S蛋白酶体浓度均升高（p<0.001），且与体外循环开始60分钟时HSP 27、70和90α的血清含量显著相关（p<0.001）。CABG手术后比较白细胞介素-6水平与HSP和20S蛋白酶体的血管内渗漏情况时未发现相关性。
结论
我们从数据中得出结论，CABG手术后已知的免疫调节和凋亡相关蛋白泄漏导致先天免疫系统被激活。
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