Kato Koichi, Sasakawa Hiroaki, Kamiya Yukiko, Utsumi Maho, Nakano Michiko, Takahashi Noriko, Yamaguchi Yoshiki
Institute for Molecular Science, National Institutes of Natural Sciences, 5-1 Higashiyama Myodaiji, Okazaki 444-8787, Japan.
Biochim Biophys Acta. 2008 Mar;1780(3):619-25. doi: 10.1016/j.bbagen.2007.11.014. Epub 2007 Dec 5.
Although NMR spectroscopy has great potential to provide us with detailed structural information on oligosaccharides and glycoconjugates, the carbohydrate NMR analyses have been hampered by the severe spectral overlapping and the insufficiency of the conformational restraints. Recently, ultra-high field NMR spectrometers have become available for applications to structural analyses of biological macromolecules. Here we demonstrate that ultra-high fields offer not only increases in sensitivity and chemical shift dispersion but also potential benefits for providing unique information on chemical exchange and relaxation, by displaying NMR spectral data of oligosaccharide, glycoprotein, and glycolipid systems recorded at a 21.6 T magnetic field (corresponding to 920 MHz (1)H observation frequency). The ultra-high field NMR spectroscopy combined with sugar library and stable-isotope labeling approaches will open new horizons in structural glycobiology.
尽管核磁共振光谱法在为我们提供关于寡糖和糖缀合物的详细结构信息方面具有巨大潜力，但碳水化合物的核磁共振分析一直受到严重的谱线重叠和构象限制不足的阻碍。最近，超高场核磁共振光谱仪已可用于生物大分子的结构分析。在此，我们通过展示在21.6 T磁场（对应于920 MHz的¹H观测频率）下记录的寡糖、糖蛋白和糖脂系统的核磁共振光谱数据，证明超高场不仅提高了灵敏度和化学位移分散度，还为提供有关化学交换和弛豫的独特信息带来了潜在益处。超高场核磁共振光谱法与糖库和稳定同位素标记方法相结合，将为结构糖生物学开辟新的视野。
