光动力疗法对浮游培养中变形链球菌活力的影响。
The impact of photodynamic therapy on the viability of Streptococcus mutans in a planktonic culture.
作者信息
Bevilacqua I M, Nicolau R A, Khouri S, Brugnera A, Teodoro G R, Zângaro R A, Pacheco M T T
机构信息
Health and Sciences College, Vale do Paraíba University (UNIVAP), Urbanova, São José dos Campos, Brazil.
出版信息
Photomed Laser Surg. 2007 Dec;25(6):513-8. doi: 10.1089/pho.2007.2109.
OBJECTIVE
This study investigated the effect of photodynamic therapy (PDT) with toluidine blue O (TBO) and a light-emitting diode (LED) on the viability of Streptococcus mutans cells in a planktonic culture.
BACKGROUND DATA
Growth of Streptococcus mutans is the first step in the development of tooth decay. The use of light and dyes promotes cellular death in a noninvasive way, reducing treatment time.
METHODS
The LED used in this study had output power of 116 mW, its energy was 21 J, and the fluency was 2.18 J/cm(2). Samples were prepared and divided into five groups: (1) control group (-); (2) control group (+); (3) TBO; (4) LED; and (5) LED + TBO.
RESULTS
One hundred percent of the bacteria were killed following irradiation with LED and TBO. The biofilm that formed on the glass surfaces was analyzed by SEM and colony count.
CONCLUSIONS
It was demonstrated that PDT was efficient at killing microorganisms and preventing the formation of biofilms.
目的
本研究调查了用甲苯胺蓝O（TBO）和发光二极管（LED）进行光动力疗法（PDT）对浮游培养的变形链球菌细胞活力的影响。
背景数据
变形链球菌的生长是龋齿发展的第一步。光和染料的使用以非侵入性方式促进细胞死亡，减少治疗时间。
方法
本研究中使用的LED输出功率为116 mW，能量为21 J，通量为2.18 J/cm²。制备样本并分为五组：（1）对照组（-）；（2）对照组（+）；（3）TBO组；（4）LED组；（5）LED + TBO组。
结果
用LED和TBO照射后100%的细菌被杀死。通过扫描电子显微镜（SEM）和菌落计数分析在玻璃表面形成的生物膜。
结论
结果表明光动力疗法在杀死微生物和防止生物膜形成方面是有效的。
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