Wacogne Bruno, Pieralli Christian, Roux Christophe, Gharbi Tijani
Département d'Optique P.M Duffieux, Institut FEMTO-ST U.M.R CNRS no. 6174, Université de Franche-Comté, 25030, Besancon cedex, France.
Biomed Microdevices. 2008 Jun;10(3):411-9. doi: 10.1007/s10544-007-9150-7.
The elasticity of human oocytes has been estimated by means of a very simple SU-8 microsystem. The device consists of a double SU-8 layer spin coated on a silicon wafer. A micro-beam is patterned by standard photolithography. The silicon wafer is back etched using deep reactive ion etching (DRIE) in order to release the micro-beam. Because the SU-8 resin exhibits a very low Young modulus, beams with stiffnesses of 12 N/m have been easily fabricated. Although the stiffness is not as low as that of Atomic Force Microscopes cantilevers, it was possible to estimate the "deformation-load" characteristics of a few oocytes. A "flattening parameter" of the oocytes is proposed as a function of the applied load and experimental values range from 0.86 microm(2)/Pa to 3.4 microm(2)/Pa for the oocytes under test. Note that this paper belongs to the very few communications concerning the mechanical behaviour of human oocytes.
人类卵母细胞的弹性已通过一个非常简单的SU-8微系统进行了估算。该装置由旋涂在硅片上的双层SU-8层组成。通过标准光刻技术制作微梁。使用深反应离子刻蚀（DRIE）对硅片进行背面刻蚀，以释放微梁。由于SU-8树脂的杨氏模量非常低，因此很容易制造出刚度为12 N/m的梁。尽管该刚度不如原子力显微镜悬臂的刚度低，但仍有可能估算出一些卵母细胞的“变形-负载”特性。提出了卵母细胞的“扁平化参数”作为所施加负载的函数，对于受试卵母细胞，实验值范围为0.86 微米²/帕至3.4 微米²/帕。请注意，本文属于极少数关于人类卵母细胞力学行为的通信。
