Imperio Tatiana, Viti Carlo, Marri Laura
Dipartimento di Biologia Evolutiva, Università degli Studi di Siena, Via A. Moro 2, 53100 Siena, Italy.
Int J Syst Evol Microbiol. 2008 Jan;58(Pt 1):221-5. doi: 10.1099/ijs.0.65092-0.
Gram-positive, rod-shaped, endospore-forming, thermophilic bacteria were isolated from a geothermal soil collected on the north-west slope of Mount Melbourne in Antarctica. They grew aerobically at 42-60 degrees C (optimum 55 degrees C) and at pH 4.5-7.5 (optimum pH 5.5). Phylogenetic analysis of 16S rRNA gene sequences showed that these isolates were related most closely to the type strain of Alicyclobacillus pomorum (91% similarity). Growth occurred in the presence of ferrous iron at micromolar concentrations and acid was produced from various sugars. Iso-branched fatty acids C(15:0) (45.56%) and C(17:0) (35.81%) were the most abundant cellular fatty acids. The DNA G+C content was 55.1 mol%. On the basis of phenotypic and phylogenetic characteristics, it is concluded that these strains represent a novel species of the genus Alicyclobacillus, for which the name Alicyclobacillus pohliae sp. nov. is proposed. The type strain is MP4(T) (=CIP 109385(T) =NCIMB 14276(T)).
从南极洲墨尔本山北坡采集的地热土壤中分离出革兰氏阳性、杆状、产芽孢的嗜热细菌。它们在42 - 60℃（最适温度55℃）需氧生长，pH值为4.5 - 7.5（最适pH值5.5）。对16S rRNA基因序列的系统发育分析表明，这些分离株与嗜热栖热放线菌模式菌株关系最为密切（相似度为91%）。在微摩尔浓度的亚铁存在下生长，并能利用多种糖类产酸。异支链脂肪酸C(15:0)（45.56%）和C(17:0)（35.81%）是最丰富的细胞脂肪酸。DNA G + C含量为55.1 mol%。根据表型和系统发育特征，得出这些菌株代表嗜热栖热放线菌属的一个新物种的结论，为此提出新名称嗜热栖热放线菌波氏新种。模式菌株为MP4(T)（=CIP 109385(T) =NCIMB 14276(T)）。
