Guo Xu, You Xiao-Yan, Liu Li-Jun, Zhang Jia-Yue, Liu Shuang-Jiang, Jiang Cheng-Ying
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Int J Syst Evol Microbiol. 2009 Oct;59(Pt 10):2415-20. doi: 10.1099/ijs.0.008870-0. Epub 2009 Jul 21.
A novel mesophilic, acidophilic, endospore-forming bacterium, designated strain ZJ-6(T), was isolated from Zi-Jin copper mine in Inner Mongolia, China. Cells of strain ZJ-6(T) were rod-shaped, stained Gram-positive or were Gram-variable, and grew aerobically at 25-35 degrees C (optimum, 30 degrees C) and pH 2.0-6.0 (optimum, pH 3.5). 16S rRNA gene sequence analysis showed that strain ZJ-6(T) was related phylogenetically to members of the genus Alicyclobacillus, with 16S rRNA gene sequence similarities of 89.5-94.2 %. Cells contained MK-7 as the major quinone and the DNA G+C content was 51.2 mol%. Strain ZJ-6(T) possessed a number of phenotypic characteristics that differentiated it from recognized Alicyclobacillus species, including its growth temperature, assimilation of various carbon sources, production of acids from a range of compounds, and the ability to grow chemoautotrophically using ferrous iron, elemental sulfur and tetrathionate as electron donors. The predominant cellular fatty acids of strain ZJ-6(T) were anteiso-C(15 : 0) (67.1 %), iso-C(16 : 0) (7.7 %) and anteiso-C(17 : 0) (7.4 %); omega-alicyclic fatty acids were not found. On the basis of these results, it is concluded that strain ZJ-6(T) represents a novel species within the genus Alicyclobacillus, for which the name Alicyclobacillus aeris sp. nov. is proposed; the type strain is ZJ-6(T) (=CGMCC 1.7072(T)=NBRC 104953(T)).
从中国内蒙古紫金铜矿分离出一株新型嗜温、嗜酸、产芽孢细菌，命名为ZJ-6(T)菌株。ZJ-6(T)菌株的细胞呈杆状，革兰氏染色呈阳性或革兰氏可变，在25-35℃（最适温度30℃）和pH 2.0-6.0（最适pH 3.5）条件下好氧生长。16S rRNA基因序列分析表明，ZJ-6(T)菌株在系统发育上与 Alicyclobacillus属的成员相关，16S rRNA基因序列相似性为89.5-94.2%。细胞以MK-7作为主要醌，DNA的G+C含量为51.2 mol%。ZJ-6(T)菌株具有许多表型特征，使其与已确认的 Alicyclobacillus属物种不同，包括其生长温度、对各种碳源的同化作用、从一系列化合物中产酸的能力以及利用亚铁、元素硫和连四硫酸盐作为电子供体进行化能自养生长的能力。ZJ-6(T)菌株的主要细胞脂肪酸为anteiso-C(15 : 0)（67.1%）、iso-C(16 : 0)（7.7%）和anteiso-C(17 : 0)（7.4%）；未发现ω-脂环族脂肪酸。基于这些结果，得出结论：ZJ-6(T)菌株代表 Alicyclobacillus属内的一个新物种，为此提出名为Alicyclobacillus aeris sp. nov.的名称；模式菌株为ZJ-6(T)（=CGMCC 1.7072(T)=NBRC 104953(T)）。
