Graduate School of Engineering, Tokyo Denki University, 5, Senju asahi-cho, Adachi-ku, Tokyo, 120-8551, Japan.
National Institute of Technology and Evaluation (NITE), 2-5-8, Kazusa-kamatari, Kisarazu, Chiba, Japan.
Arch Microbiol. 2021 Oct;203(8):4859-4865. doi: 10.1007/s00203-021-02470-9. Epub 2021 Jul 7.
A Gram-positive, rod-shaped, spore-forming, thermophilic, and acidophilic bacterium, designated as strain skT53, was isolated from farm soil in Tokyo, Japan. Under aerobic conditions, the strain grew at 35-55 °C (optimum temperature 44-55 °C) and pH 4.0-6.0 (optimum pH 5.0). Phylogenetic analysis of the 16S rRNA gene sequence showed that the isolate was moderately related to the type strain of Effusibacillus consociatus (94.3% similarity). The G + C content of the genomic DNA was 48.2 mol%, and MK-7 was the predominant respiratory quinone in the strain. The major fatty acids were anteiso-C, iso-C, and iso-C. Based on the phenotypic and chemotaxonomic characteristics, as well as 16S rRNA gene sequence similarity and whole genome analyses, strain skT53 represents a novel species in the genus Effusibacillus, for which the name Effusibacillus dendaii sp. nov. has been proposed. The type strain is skT53 (= NBRC 114101 = TBRC 11241).
一株革兰氏阳性、杆状、产孢、嗜热、嗜酸的细菌，被命名为 skT53 菌株，从日本东京的农田土壤中分离得到。在好氧条件下，该菌株在 35-55°C（最佳温度 44-55°C）和 pH4.0-6.0（最佳 pH5.0）的条件下生长。16S rRNA 基因序列的系统发育分析表明，该分离株与协同埃弗拉塔斯氏菌（Effusibacillus consociatus）的模式株中度相关（相似度为 94.3%）。基因组 DNA 的 G+C 含量为 48.2mol%，菌株中的主要呼吸醌为 MK-7。主要脂肪酸为 anteiso-C、iso-C 和 iso-C。基于表型和化学分类特征以及 16S rRNA 基因序列相似性和全基因组分析，菌株 skT53 代表埃弗拉塔斯氏菌属中的一个新种，现提议将其命名为 dendaii 埃弗拉塔斯菌（Effusibacillus dendaii）。模式菌株为 skT53（=NBRC 114101=TBRC 11241）。
