Chien Chia-Chi, Liu Kuan-Ting, Duh Jenq-Gong, Chang Kuo-Wei, Chung Kwok-Hung
Institute of Oral Biology, National Yang-Ming University, Taipei, Taiwan.
Dent Mater. 2008 Jul;24(7):986-93. doi: 10.1016/j.dental.2007.11.020. Epub 2008 Feb 21.
To evaluate the cytotoxicity of nickel-based alloy surfaces after nitride film coatings.
A total of 120 disc-shaped specimens (1.5 x 12.0mm) were prepared from nickel (Ni) alloy ingots and metallurgically ground with silicon carbide (SiC) sandpaper to 1200 grit and used as the ground group. Ninety specimens from the ground group were selected and further polished with 1.0 microm aluminum powder slurry and assigned as the polished group. Titanium nitride (TiN) and titanium-aluminum nitride (TiAlN) film coatings were deposited onto 30 polished specimens each by a reactive radio frequency magnetron sputter deposition system and used as coated groups, respectively. The morphological changes and cytoskeleton of tested human gingival fibroblasts were observed using fluorescence microscopy at 3h and 24h time periods, respectively. An MTT assay was used to assess cell viability at 24h. The results were statistically analyzed (n=5, ANOVA, Scheffe', p<0.05).
After 3h of incubation, cells began to spread on the test surfaces. Spindle-shaped fibroblasts with well-developed cytoskeleton and distinct actin fibers were observed at the 24h incubation point on the polished and coated specimens. Results of the MTT assay revealed that the TiN and TiAlN film coated groups were significantly higher in cell proliferation and viability than the polished and control groups (p<0.05).
The biocompatibility of Ni-based alloy was increased significantly after nitride film coating.
评估氮化膜涂层后镍基合金表面的细胞毒性。
从镍（Ni）合金铸锭制备120个圆盘形试样（1.5×12.0mm），用碳化硅（SiC）砂纸进行金相研磨至1200目，作为研磨组。从研磨组中选取90个试样，用1.0微米铝粉浆料进一步抛光，作为抛光组。通过反应射频磁控溅射沉积系统分别在30个抛光试样上沉积氮化钛（TiN）和钛铝氮化物（TiAlN）薄膜涂层，分别作为涂层组。分别在3小时和24小时时间段使用荧光显微镜观察受试人牙龈成纤维细胞的形态变化和细胞骨架。采用MTT法评估24小时时的细胞活力。对结果进行统计学分析（n = 5，方差分析，谢费检验，p < 0.05）。
孵育3小时后，细胞开始在测试表面铺展。在抛光和涂层试样上孵育24小时时，观察到具有发达细胞骨架和明显肌动蛋白纤维的纺锤形成纤维细胞。MTT分析结果显示，TiN和TiAlN薄膜涂层组的细胞增殖和活力明显高于抛光组和对照组（p < 0.05）。
氮化膜涂层后镍基合金的生物相容性显著提高。
