Cilurzo Francesco, Minghetti Paola, Sinico Chiara
Istituto di Chimica Farmaceutica e Tossicologica - P. Pratesi, Università degli Studi di Milano, Viale Abruzzi, 42 - 20131 Milan, Italy.
AAPS PharmSciTech. 2007 Nov 9;8(4):E94. doi: 10.1208/pt0804094.
The suitability of newborn pig skin as an alternative to human epidermis in in vitro permeation studies was investigated. A set of 7 benzoxazinones was used to perform in vitro experiments by using a modified Franz diffusion cell and excised newborn pig skin as a membrane. The maximum flux through newborn pig skin () was compared with the maximum flux through excised human epidermis (), available from the literature, by means of the factor of difference value FoD=/J. The FoD values ranged from 0.48 to 1.91, indicating that and were in the same order of magnitude. This result confirmed the suitability of this membrane to assess the permeability of not completely freely water soluble drugs, such as the set of benzoxazinones used in the present study and propranolol hydrochloride. Considering that the skin was withdrawn from animals that died of natural causes, the ethical problems connected with the use of animal skin in preliminary permeation screenings can be bypassed.
研究了新生猪皮在体外渗透研究中作为人表皮替代品的适用性。使用一组7种苯并恶嗪酮，通过改良的Franz扩散池并以切除的新生猪皮作为膜进行体外实验。通过差异值因子FoD = J/J，将通过新生猪皮的最大通量(J)与文献中可得的通过切除的人表皮的最大通量(J)进行比较。FoD值范围为0.48至1.91，表明J和J处于相同数量级。该结果证实了这种膜适用于评估并非完全自由水溶性药物的渗透性，例如本研究中使用的苯并恶嗪酮组和盐酸普萘洛尔。考虑到皮肤取自自然死亡的动物，在初步渗透筛选中使用动物皮肤所涉及的伦理问题可以避免。
