Kanpipit Nattawadee, Nualkaew Natsajee, Thapphasaraphong Suthasinee
Biomedical Science Program, Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand.
Pharmaceuticals (Basel). 2022 Dec 27;16(1):35. doi: 10.3390/ph16010035.
Sericin-hydrogel formulations incorporating purple waxy corn ( L.) cob extract (PWCC) were developed as potential topical skin cosmetic products. Sericin has wound healing properties, protects against ultraviolet (UV) radiation, and exhibits anti-inflammatory, anti-oxidation, and anti-tyrosinase activities. PWCC is a rich source of anthocyanins with antioxidants, UV protective, anti-inflammatory, and collagen-enhancing activities. Six hydrogel formulations (S1-S6) were investigated for anti-melanogenesis on the B16F10 melanoma cell line and UV-protection on human keratinocytes (HaCaT) and anti-aging activities on normal human dermal fibroblasts (NHDFs). The results showed that the hydrogel formulations enhanced the anthocyanin permeation through the skin. The S4 formulation indicated the highest inhibition of tyrosinase activity and reduced the melanin pigment, increased the cell viability of the UV-induced HaCaT cells, the inhibition of collagenase and elastase, and increased the collagen type I production without cytotoxicity. Therefore, the PWCC loaded-sericin hydrogels show a high potential as a novel anti-hyperpigmentation, UV protection, and anti-aging products for topical applications.
含有紫糯玉米（L.）芯提取物（PWCC）的丝胶蛋白水凝胶制剂被开发为潜在的局部皮肤美容产品。丝胶蛋白具有伤口愈合特性，能抵御紫外线（UV）辐射，并具有抗炎、抗氧化和抗酪氨酸酶活性。PWCC富含花青素，具有抗氧化、紫外线防护、抗炎和增强胶原蛋白的活性。研究了六种水凝胶制剂（S1 - S6）对B16F10黑色素瘤细胞系的抗黑色素生成作用、对人角质形成细胞（HaCaT）的紫外线防护作用以及对正常人皮肤成纤维细胞（NHDFs）的抗衰老作用。结果表明，水凝胶制剂增强了花青素透过皮肤的渗透。S4制剂显示出对酪氨酸酶活性的最高抑制作用，减少了黑色素色素，提高了紫外线诱导的HaCaT细胞的细胞活力，抑制了胶原酶和弹性蛋白酶，并增加了I型胶原蛋白的产生，且无细胞毒性。因此，负载PWCC的丝胶蛋白水凝胶作为新型的局部应用抗色素沉着、紫外线防护和抗衰老产品具有很高的潜力。
