嗜热栖热菌中古菌操纵子转录的极性
Polarity in archaeal operon transcription in Thermococcus kodakaraensis.
作者信息
Santangelo Thomas J, Cubonová L'ubomíra, Matsumi Rie, Atomi Haruyuki, Imanaka Tadayuki, Reeve John N
机构信息
Department of Microbiology, Ohio State University, Columbus, OH 43210-1292, USA.
出版信息
J Bacteriol. 2008 Mar;190(6):2244-8. doi: 10.1128/JB.01811-07. Epub 2008 Jan 11.
Abstract
An in vivo archaeal gene reporter system has been established based on TK1761, a gene that encodes a nonessential beta-glycosidase in Thermococcus kodakaraensis. Following the introduction of nonsense codons into promoter-proximal genes, polarity in operon expression in this archaeon has been established by both microarray hybridization assays and a reporter gene expression system.
摘要
基于TK1761建立了一种体内古菌基因报告系统，TK1761是一种编码嗜热栖热菌中非必需β-糖苷酶的基因。在启动子近端基因中引入无义密码子后，通过微阵列杂交分析和报告基因表达系统确定了该古菌操纵子表达中的极性。
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