Roperto S, Borzacchiello G, Brun R, Perillo A, Russo V, Urraro C, Roperto F
Department of Pathology and Animal Health, Faculty of Veterinary Medicine, Naples University Federico II, Naples, Italy.
Vet Pathol. 2008 Jan;45(1):39-42. doi: 10.1354/vp.45-1-39.
We report a case of multiple glomus tumors associated with bovine papillomavirus type 2 (BPV-2) infection in the urinary bladder of a 13-year-old cow suffering from severe chronic enzootic hematuria. Macroscopically, multiple submucosal reddish nodules were seen swelling the vesical mucosa. Histologically, neoplastic proliferation was characterized by the presence of numerous blood vessels. These were lined by normal endothelial cells surrounded by round epithelioid cells with central nuclei, prominent nucleoli, acidophilic cytoplasm, and well-defined cytoplasmic borders. Tumor cells were distributed around open vascular lumina and in perivascular spaces. They were immunohistochemically positive for actin and vimentin and negative for cytokeratins, desmin, and factor VIII-related antigen. On the basis of these findings, this tumor was diagnosed as glomus tumor, a neoplasm not previously reported in cattle and exceedingly rare in animals. BPV-2 DNA was amplified from the formalin-fixed, paraffin-processed tissue specimens obtained by laser capture microdissection. This report widens the spectrum of mesenchymal tumors of the bovine urinary bladder. Finally, the microscopic pattern of tumor described here shares striking morphologic and immunohistochemical similarities with the angiomatous form of glomus tumor known to occur in man.
我们报告了一例13岁患有严重慢性地方流行性血尿的奶牛膀胱中与2型牛乳头瘤病毒（BPV-2）感染相关的多发性球旁细胞瘤。宏观上，可见多个黏膜下红色结节使膀胱黏膜肿胀。组织学上，肿瘤性增殖的特征是存在大量血管。这些血管由正常内皮细胞衬里，周围是圆形上皮样细胞，细胞核居中，核仁突出，细胞质嗜酸性，细胞质边界清晰。肿瘤细胞分布在开放的血管腔周围和血管周围间隙。它们免疫组化检测肌动蛋白和波形蛋白呈阳性，细胞角蛋白、结蛋白和因子VIII相关抗原呈阴性。基于这些发现，该肿瘤被诊断为球旁细胞瘤，这是一种此前未在牛中报道且在动物中极为罕见的肿瘤。通过激光捕获显微切割从福尔马林固定、石蜡包埋的组织标本中扩增出BPV-2 DNA。本报告拓宽了牛膀胱间叶性肿瘤的范围。最后，此处描述的肿瘤微观模式与已知发生在人类的血管瘤样球旁细胞瘤在形态学和免疫组化方面具有显著相似性。
