Seeberger Peter H
Laboratory for Organic Chemistry, Swiss Federal Institute of Technology (ETH) Zürich, HCI F 315, Wolfgang-Pauli-Str. 10, CH-8093 Zürich, Switzerland.
Chem Soc Rev. 2008 Jan;37(1):19-28. doi: 10.1039/b511197h. Epub 2007 Aug 20.
Peptides and oligonucleotides are prepared by automated synthesizers that can be operated by non-specialists. Carbohydrates have been hard to assemble, but the increasing awareness of the biological importance of this class of complex repeating biopolymers has prompted efforts to accelerate their synthesis. This tutorial review defines the state of the art of automated solid phase oligosaccharide synthesis and identifies areas in need of further innovation. Application of the automated synthesis method to prepare a malaria vaccine candidate is briefly highlighted.
肽和寡核苷酸由非专业人员即可操作的自动合成仪制备。碳水化合物一直难以组装，但人们对这类复杂重复生物聚合物的生物学重要性的认识不断提高，促使人们努力加速其合成。本教程综述定义了自动固相寡糖合成的技术现状，并确定了需要进一步创新的领域。简要介绍了应用自动合成方法制备一种疟疾疫苗候选物的情况。
