Tokatlidou Despina, Tsivitanidou Maria, Pournaras Spyros, Ikonomidis Alexandros, Tsakris Athanassios, Sofianou Danae
Department of Clinical Microbiology, Hippokration General Hospital, Thessaloniki, Greece.
J Clin Microbiol. 2008 Mar;46(3):1005-8. doi: 10.1128/JCM.01573-07. Epub 2008 Jan 16.
From November 2006 to April 2007, nine nonrepetitive isolates of Klebsiella pneumoniae with reduced susceptibility or resistance to carbapenems were recovered from clinical specimens from separate patients hospitalized in a tertiary care hospital. The imipenem-EDTA synergy test was positive for all isolates. PCR, sequencing, and transferability experiments revealed the novel bla(VIM-12) metallo-beta-lactamase gene, which was plasmid mediated and located in a class 1 integron. Pulsed-field gel electrophoresis demonstrated a single macrorestriction pattern, indicating the clonal spread of VIM-12-producing K. pneumoniae.
2006年11月至2007年4月,从一家三级护理医院收治的不同患者的临床标本中分离出9株对碳青霉烯类药物敏感性降低或耐药的非重复性肺炎克雷伯菌菌株。所有分离株的亚胺培南-EDTA协同试验均为阳性。聚合酶链反应(PCR)、测序和可转移性实验揭示了新型bla(VIM-12)金属β-内酰胺酶基因,该基因由质粒介导,位于1类整合子中。脉冲场凝胶电泳显示单一的宏观限制性图谱,表明产VIM-12的肺炎克雷伯菌存在克隆传播。