Jeyaratnam Dakshika, Gottlieb Anna, Ajoku Uchechukwu, French Gary L
Department of Infection, Infection and Immunology Delivery Unit, Guy's and St Thomas' Hospital NHS Foundation Trust, St Thomas' Hospital, London SE1 7EH, UK.
Diagn Microbiol Infect Dis. 2008 May;61(1):1-5. doi: 10.1016/j.diagmicrobio.2007.12.004. Epub 2008 Jan 16.
A commercial rapid polymerase chain reaction methicillin-resistant Staphylococcus aureus (MRSA) screening method (IDI-MRSA) is validated for the use with nasal swabs transported in liquid Stuart's medium. We investigated the use of IDI-MRSA for screening for MRSA in pooled nose, axilla, and groin swabs and in single swabs from skin puncture sites, wounds, throat, rectum, and groin using swabs transported in Amies medium without charcoal. We performed the IDI-MRSA test on swabs that had been used for routine MRSA broth culture and which were selected to be about 50% MRSA positive. We compared the IDI-MRSA result with the MRSA culture result. With 201 pooled sets, the sensitivity of IDI-MRSA was 85% and the specificity 95%. With 32 single screening swabs, sensitivity was 94% and specificity 80%. The method is not compromised by swab transport in Amies medium if an additional heating step is used. We had a low rate of initial inhibition (1.3%), which may have been due to the extra heating step used to liquefy gel from the Amies medium. Thus, in this study IDI-MRSA gives similar results to culture with pooled or single swabs from multiple screening sites.
一种商业快速聚合酶链反应耐甲氧西林金黄色葡萄球菌（MRSA）筛查方法（IDI-MRSA）已被验证可用于在液体斯图尔特培养基中运输的鼻拭子。我们研究了IDI-MRSA用于在合并的鼻、腋窝和腹股沟拭子以及来自皮肤穿刺部位、伤口、喉咙、直肠和腹股沟的单个拭子中筛查MRSA的情况，这些拭子使用不含活性炭的阿姆斯培养基运输。我们对已用于常规MRSA肉汤培养且被选择为约50% MRSA阳性的拭子进行了IDI-MRSA测试。我们将IDI-MRSA结果与MRSA培养结果进行了比较。对于201组合并拭子，IDI-MRSA的敏感性为85%，特异性为95%。对于32个单筛拭子，敏感性为94%，特异性为80%。如果使用额外的加热步骤，该方法不会因在阿姆斯培养基中运输拭子而受到影响。我们的初始抑制率较低（1.3%），这可能是由于用于从阿姆斯培养基中液化凝胶的额外加热步骤所致。因此，在本研究中，IDI-MRSA与来自多个筛查部位的合并或单个拭子培养结果相似。
