Health Protection Agency London Region Laboratory, Department of Medical Microbiology, Kings College Hospital, Denmark Hill, London SE5 9RS, United Kingdom.
J Clin Microbiol. 2010 Dec;48(12):4559-62. doi: 10.1128/JCM.01512-10. Epub 2010 Oct 20.
As health services move toward universal methicillin-resistant Staphylococcus aureus (MRSA) screening for hospital admissions, the most cost-effective approach is yet to be defined. In this study, one of the largest to date, we evaluated the performance of the BD GeneOhm MRSA assay on the Rotor-Gene 6000 thermal cycler, using samples taken directly from pooled MRSA screens. Results were compared with the same assay performed on the Smart-Cycler II platform and overnight broth culture. Samples yielding discrepant results were subjected to detailed analysis with an in-house PCR and patient note review. A total of 1,428 pooled MRSA screens were tested. Sensitivities and specificities of 85.3% and 95.8% for the Rotor-Gene and 81% and 95.7% for the Smart-Cycler were obtained, compared with broth enrichment. The sensitivity of the BD GeneOhm assay was increased to 100% when the results of in-house PCR and patient note review were taken into account. This study demonstrates that the Rotor-Gene 6000 thermal cycler is a reliable platform for use with the BD GeneOhm assay. It also proves that commercial PCR can be performed direct on pooled samples in selective broth, without the need for overnight incubation.
随着医疗服务向医院入院时普遍进行耐甲氧西林金黄色葡萄球菌（MRSA）筛查的方向发展，最具成本效益的方法尚待确定。在这项迄今为止规模最大的研究之一中，我们评估了 BD GeneOhm MRSA 检测在 Rotor-Gene 6000 热循环仪上的性能，使用直接从 MRSA 筛查的混合样本进行检测。结果与在 Smart-Cycler II 平台和过夜肉汤培养物上进行的相同检测进行了比较。对产生不一致结果的样本进行了详细分析，包括内部 PCR 和患者记录审查。共测试了 1428 个混合 MRSA 筛查样本。与肉汤富集相比，Rotor-Gene 和 Smart-Cycler 的灵敏度和特异性分别为 85.3%和 95.8%以及 81%和 95.7%。当考虑内部 PCR 和患者记录审查的结果时，BD GeneOhm 检测的灵敏度提高到 100%。这项研究表明 Rotor-Gene 6000 热循环仪是与 BD GeneOhm 检测配合使用的可靠平台。它还证明商业 PCR 可以直接在选择性肉汤中的混合样本上进行，而无需过夜孵育。
