长5'前导序列对真核生物核糖体体外起始作用的影响。
Effects of long 5' leader sequences on initiation by eukaryotic ribosomes in vitro.
作者信息
Kozak M
机构信息
Department of Biochemistry, University of Medicine and Dentistry of New Jersey, Piscataway 08854.
出版信息
Gene Expr. 1991 May;1(2):117-25.
Abstract
Lengthening the 5' noncoding sequence on SP6-derived transcripts can increase their translational efficiency by an order of magnitude under some conditions of translation in reticulocyte lysates. This effect was observed upon reiterating three different synthetic oligonucleotides, the sequences of which were designed simply to preclude secondary structure. It seems unlikely that such arbitrarily designed sequences are recognized by sequence-specific translational enhancer proteins. Rather, long 5' leader sequences appear to accumulate extra 40S ribosomal subunits, which may account for their translational advantage. The buildup of 40S subunits on long, unstructured leader sequences is predicted by the scanning model for initiation. Leader sequences such as these may be ideal for in vitro expression vectors.
摘要
在网织红细胞裂解物的某些翻译条件下，延长SP6衍生转录本的5'非编码序列可使其翻译效率提高一个数量级。在重复三种不同的合成寡核苷酸时观察到了这种效应，其序列设计仅为了防止二级结构形成。这种任意设计的序列似乎不太可能被序列特异性翻译增强蛋白识别。相反，长的5'前导序列似乎会积累额外的40S核糖体亚基，这可能是它们具有翻译优势的原因。起始的扫描模型预测，长的无结构前导序列上会积累40S亚基。这样的前导序列可能是体外表达载体的理想选择。
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