Brendler T, Godefroy-Colburn T, Carlill R D, Thach R E
J Biol Chem. 1981 Nov 25;256(22):11747-54.
Competition among excess mRNAs for a limiting, message-discriminatory initiation component (or set of components) has been studied in vitro. Procedures have been devised which provide maximum sensitivity with minimum potential for artifact. These procedures produce data that can be analyzed quantitatively to obtain numerical constants characteristic of a given mRNA's intrinsic initiation efficiency or, more precisely, competitive efficiency. A major advantage of this approach is that this information can be determined for individual mRNAs within a mixture of RNAs without the necessity of determining individual mRNA concentrations. The effects of potassium chloride concentration and magnesium acetate concentration on mRNA competitive efficiencies have been studied using these procedures.
体外研究了过量信使核糖核酸（mRNA）对有限的、具有信息识别能力的起始成分（或一组成分）的竞争情况。已设计出一些程序，这些程序能以最小的人为干扰可能性提供最大的灵敏度。这些程序产生的数据可进行定量分析，以获得给定mRNA内在起始效率的数值常数，或者更准确地说，是竞争效率的数值常数。这种方法的一个主要优点是，可以在不测定单个mRNA浓度的情况下，确定RNA混合物中单个mRNA的这些信息。利用这些程序研究了氯化钾浓度和醋酸镁浓度对mRNA竞争效率的影响。
